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BIOD 171 FINAL EXAM LATEST EXAM | ALL QUESTIONS AND ANSWERS WITH DETAILED EXPLANATIONS | GRADED A+ | VERIFIED ANSWERS | JUST RELEASED$22.99
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BIOD 171 FINAL EXAM LATEST EXAM | ALL
QUESTIONS AND ANSWERS WITH
DETAILED EXPLANATIONS | GRADED A+ |
VERIFIED ANSWERS | JUST RELEASED
You want to observe the size and shape of a cell. What is the easiest
staining technique that you could perform? Name at least one dye you
would use during this process. ------CORRECT ANSWER---------------- A
simple stain
- crystal violet dye.
Simple stain. You could use any of the following: methylene blue, crystal
violet, safranin or fuschin.
You want to observe the movement of microorganisms that are present in a
sample of cheek cells obtained on a patient. Briefly explain the technique
that you would use and how it is performed. ------CORRECT ANSWER------
---------A wet mount stain is indicated. A small liquid culture (usually just a
drop) containing a microorganism of interest is prepared, added to a slide,
and then covered with a glass coverslip. The coverslip is present to both
protect the objective and the specimen while also holding the
microorganism in place. Wet mounts are used to observe the motility and
behavior of an organism.
You suspect a patient may have TB. Once a sample has been obtained, it
is sent off to the lab for an acid-fast stain. If the patient were infected with
TB, describe what you would expect to see on the stained slide. ------
CORRECT ANSWER---------------Red cells on a blue background.
You would expect to see red cells (TB+) on a blue background (TB
negative).
,True or False: If a patient is suspected of having malaria, a Giesma stain
would be an appropriate differential test to perform. ------CORRECT
ANSWER---------------True
Identify what type of electron microscope was used to capture the following
image and explain your choice. (A) ------CORRECT ANSWER---------------
Transmission Electron Microscope (TEM) because this image shows a 2D
image of the internal structure, which is only shown by the TEM.
The above image is captured via a Transmission Electron Microscope
(TEM). Even at 20nm resolution (inset image), subcellular substructures
are still visible. The image lacks the outside 'shell' only appearance of
SEM.
True or False: A Giemsa stain can be used to determine the presence of
pathogenic bacteria. ------CORRECT ANSWER---------------True
When performing a quadrant streak, the sample is spread across the plate
in such a way to form what? ------CORRECT ANSWER---------------A dilution
gradient.
A dilution gradient is formed. The resulting gradient should always contain
within it the growth of individual colonies.
A dilution gradient is formed when carrying out what generalize plating
strategy? ------CORRECT ANSWER---------------Phase-Dilution streak
, In what phase of dilution streak would you expect to find the lowest
concentration of bacteria. P2 or P4? ------CORRECT ANSWER---------------
P4.
The phases concentration rank from P1 being the highest, then P2, P3, and
P4.
P4 (Phase 4) would contain the lowest concentration of bacteria. The
phases rank (from highest to lowest), P1 > P2 > P3 > P4.
True or False. When performing a dilution streak a new (or sterilized) loop
is not required for each phase as long as the bacterial culture is pure. ------
CORRECT ANSWER---------------False
True or False. When performing a dilution streak a new (or sterilized) loop
must be used for each phase. ------CORRECT ANSWER---------------True
Failure to do so would prevent the establishment of a dilution gradient, as
the same bacterial concentration would be spread across both phase
regions.
The number of phases (3 vs. 4) and/or the number of times a loop passes
through a previous phase (once vs. multiple) is acceptable provided what
happens? ------CORRECT ANSWER---------------The resulting gradient has
growth of the individual colonies in it.
Either deviation is acceptable in practice provided the resulting gradient
contains within it the growth of individual colonies—if not, the experiment
must be repeated.
True or False. To restrict the growth of a pathogenic microbe a researcher
might decrease an incubator from 37°C to 25°C. ------CORRECT
ANSWER---------------True
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