BIO 198 Lab Practical - Final Exam Study Guide with Questions and Correct Answers

What are the different levels of protein structure? primary structure Secondary structure Tertiary structure Quaternary structure Primary structure first level of protein structure, sequence of amino acids linked together to form a polypeptide chain Secondary structure second level of protein structure, regular and recurring arrangements in space of adjacent amino acid residues in a polypeptide chain Tertiary structure third level, overall three-dimensional arrangement of its polypeptide chain in space Quaternary structure fourth level, association of several protein chains or subunits into a closely packed arrangement Relationship between enzymes, substrates, and products a substrate enters the active site of an enzyme; the reaction then occurs, converting the substrate into products and forming an enzyme products complex. The products then leave the active site of the enzyme What are some environmental factors that can affect the structure of a protein? ph and temperature What does it mean when an enzyme is denatured? causes bonds to be broken, structure to be disrupted and it stops functioning - happens when enzymes are exposed to extreme conditions (high temp or extreme ph levels) How does temperature effect the rate of peroxidase 10 to 30 degrees: activity increased slowly 30 degrees and above: rate of activity increases 70 degrees: reaches its peak How was the rate of enzyme calculated slope/time