Essay
Applied Science Unit 11 Assignment - Learning Aim D
- Course
- Institution
- Book
FULL ASSIGNMENT Unit 11 Learning Aim D - Genetic Engineering. DISTINCTION graded. References Included.
[Show more]
Connected book
Book Title:
Author(s):
- Edition:
- ISBN:
- Edition:
More summaries for
-
Applied Science Unit 11 Assignment - Learning Aim B
-
Applied Science Unit 11 Assignment - Learning Aim A
-
Unit 5: Perceptions of science. Assignment 2 P2
Seller
Follow
DelilahDevi
Content preview
Genetic EngineeringBiotechnology refers to the exploitation of biological processes for any purpose.
Genetic engineering is a specific type of biotechnology which manipulates the
genome of an organism to modify its characteristics (phenotypes). The resultant
organisms of genetic engineering are referred to with the abbreviation GMOs
(Genetically Modified Organisms).
Extraction, separation and amplification of DNA within the laboratory:
Experiment technique:
Procedures such as calibration must be carried out to obtain accurate results.
Calibration is the process of checking a measuring instrument to ensure there are
no faults that could affect the accuracy of the results. You must calibrate the
equipment being used throughout the experiment; if not, the results will have a
higher uncertainty. Each piece of equipment has a calibration method individual to
its function:
Piece of equipment Calibration method
Glassware Rinse out with distilled water to
remove any residue. Also, ensure
there are no cracks/chips because if
there is product could be lost.
2d.p scales Ensure that the scales are 00.00 when turned
on and measure a known mass to test the
accuracy of the scales.
Micropipette Change the tip of the micropipette to
an unused tip between the individual
samples so that there is no
contamination.
Also, a risk assessment of any hazardous equipment/substances is carried out for
individual experiments, but these are general safety precautions that should be
taken when working in a laboratory:
To prevent the risk of the hazard; lab coats, eye protection (safety glasses/goggles)
and rubber gloves should be worn whilst handling these chemicals. Also, avoid
using high concentrations where possible.
If physical contact with chemicals does occur, rinse the exposed area with distilled
water. If the substance is spilt on a surface, wipe it with a damp cloth (if it is an
anhydrous substance, avoid lifting dust particles into the air). If symptoms occur,
seek medical attention immediately. The symptoms are not foreseeable.
Minor scratches/cuts should be washed and have a small, sterile dressing applied to
it. For severe cuts, sit down and apply pressure to the wound using a sterile
dressing. Seek immediate medical attention. Treat burns by rinsing it with cold
water and seek medical attention for severe burns.
, DNA extraction is a method used to obtain and isolate DNA from their cells.
Genomic DNA within prokaryotic cells can be extracted from their plasmids while
DNA within eukaryotic cells can be extracted from the nucleus as well as the
mitochondria and chloroplasts if the cells have been differentially centrifuged –
differential centrifugation refers to a technique that allows the organelles of a cell to
separate based on sedimentation rates. This technique can is used to sample plant
DNA.
Differential centrifugation experiment:
Apparatus:
250ml beaker x2
25ml beaker x2
100ml beaker
Salt
2dp Mass balance
Weighing bottle
Spatula
Glass rod
Strawberry
Pestle and mortar
Hot water bath
Funnel
Filter paper
Ethanol
Risk assessment:
Apparatus / Hazard Comment Prevention
substance
Ethanol Pure: Pure ethanol: -For pure ethanol, the
Flammable -Highly flammable as a concentration in the
IDA: liquid and vapour. atmosphere should not
- -As a vapor it can catch exceed 5760 mg/m
Flammable fire above 13 degrees. -For both, the area should
-Harmful The vapour/air mixture be well ventilated.
-Health is explosive. -Be cautious when
Hazard Industrial denatured carrying/placing the
alcohol: ethanol, avoiding any
-This is used within contact.
school labs. This If ingested, no more than
ethanol contains 5% rinse the mouth and spit.
methanol which makes Do not induce vomiting.
it more hazardous than Immediately contact
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller DelilahDevi. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $10.36. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
67866 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now