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ASU BIO 340 exam 3 | Questions with 100% Correct Answers
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ASU BIO 340 exam 3 | Questions with 100%Correct Answers
What protein coats the single DNA strands near the replication fork to prevent
DNA from unwinding /back/ into a double helix? - ✔️✔️Single-strand binding
proteins
What is a primer? - ✔️✔️A short segment of DNA that acts as the starting point for
a new strand
also provides the free 3'-OH end
What does RNA //primase// do? - ✔️✔️synthesizes RNA primers so that *DNA pol*
can now extend RNA primer, starting the replication process
What is the 10 step process of DNA replication in __*Prokaryotes*__ - ✔️✔️1. DNA
unwinds at origin of replication
2. //Helicase// opens the DNA forming replication forks, (which are extended
bidirectionally)
3. //single-strand binding proteins// coat the DNA around the replication forks to
prevent the re-winding of DNA
4. //Topoisomerase// binds the region //ahead// of the replication fork to
prevent supercoiling
5. Primers synthesize RNA primase //complementary to DNA strand
6. DNA pol III starts adding nucleotides to the 3'-OH end of the primer
7.//Elongation// of both the lagging and the leading strand continues
8. RNA primers are removed by //exonuclease// activity
9. Gaps are filled by DNA pol I by adding dNTPs
,10. The gap between 2 DNA fragments is sealed by //Ligase//
Match the function with the correct enzyme (prokaryotes):
A. DNA pol I
B. DNA pol II
C. DNA pol III
1. Repair function
2. Main enzyme that adds nucleotides in the 5' to 3' direction
3. Exonuclease activity removes RNA primers and replaces with newly synthesized
DNA - ✔️✔️A->3.
B->1.
C.->2.
Match the function with the correct enzyme (prokaryotes):
A. Helicase
B. Ligase
C. Primase
1. Seals the gaps between the Okazaki fragments to create one continuous DNA
strand
2.Opens the DNA helix by breaking hydrogen bonds between nitrogenous bases
3. Synthesizes RNA primers needed to start replication - ✔️✔️A.->2.
B.->1.
C.->3.
, Match the function with the correct enzyme (prokaryotes):
A. Sliding clamp
B. Topoisomerase
C. Single-strand binding proteins
1. Binds to single-stranded DNA to avoid DNA rewinding back
2. Helps relieve the stress on DNA when unwinding by causing breaks and then
resealing the DNA
3. Helps to hold the DNA pol in place when nucleotides are being added - ✔️✔️A.-
>3
B.-> 2.
C.->1
Explain the differences between prokaryotes and eukaryotes for the following
terms:
1. Origin of replication
2. Rate of replication
3. DNA pol types
4. Telomerase
5. RNA primer removal
6. Strand elongation
7. Sliding clamp - ✔️✔️*Prokaryotes*:
1. single
2. 1000 nucleotides/second
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