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AS Biology (7401/1) Paper 1 Specimen Latest Update

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AS Biology (7401/1) Paper 1 Specimen Latest Update 01 . 1) Describe how you could use cell fractionation to isolate chloroplasts from leaf tissue. [3 marks] - ANSWER-1. How to break open cells and remove debris; 2. Solution is cold/isotonic/buffered; 3. Second pellet is chloroplast; 01 . 2) (Fi...

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  • October 12, 2024
  • 13
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • as biology 74011
  • AS Biology Paper 1 Specimen
  • AS Biology Paper 1 Specimen
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NURSING2EXAM
AS Biology (7401/1)
Paper 1 Specimen
Latest Update




01 . 1) Describe how you could use cell fractionation to isolate chloroplasts from
leaf tissue. [3 marks] - ✔✔✔ANSWER-1. How to break open cells and remove
debris;
2. Solution is cold/isotonic/buffered;
3. Second pellet is chloroplast;


01 . 2) (Figure 1 shows a photograph of a chloroplast taken with an electron
microscope.
Figure 1)




Name the parts of the chloroplast labelled A and B.
[2 marks] - ✔✔✔ANSWER-1. A stroma;

, 2. B granum;


01 . 3) Calculate the length of the chloroplast shown in Figure 1.
[1 mark] - ✔✔✔ANSWER-(length of chloroplast/length of bar)um


01 . 4) Name two structures in a eukaryotic cell that cannot be identified using an
optical
microscope.
[1 mark] - ✔✔✔ANSWER-Mitochondrion/ribosome/endoplasmic
reticulum/lysosome/cell-surface membrane


02 . 1) A technician investigated the effect of temperature on the rate of an enzyme-
controlled
reaction. At each temperature, he started the reaction using the same volume of
substrate solution and the same volume of enzyme solution.
Figure 2 shows his results.


Give one other factor the technician would have controlled.
[1 mark] - ✔✔✔ANSWER-Concentration of substrate solution / of enzyme
solution / pH;


02 . 2) Calculate the rate of reaction at 25 C.
[2 marks] - ✔✔✔ANSWER-1. 2.5/0.04;
2. g dm-3 minute-1 / g dm-3 s-1
;

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