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BMS 602 Enterobacteriaceae Exam Study Guide
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BMS 602 EnterobacteriaceaeExam Study Guide
What type of bacteria is Enterobacteriaceae - Answer Large, heterogeneous family of
medically relevant gram-negative rods
Where is Enterobacteriaceae normally found in the body - Answer Many are normal
intestinal flora of animals and humans
Are Enterobacteriaceae ubiquitous organisms? - Answer YES
Ubiquitous organisms, found worldwide in soil, water, and vegetation
What type of infections does Enterbacteriaeae cause? - Answer 30% to 35% of all
septicemias, more than 70% of urinary tract infections (UTIs), and many intestinal
infections
Enterbacteriaeae originate? - Answer Can origninate from animal or human carrier or
through endogenous spread
Is Enterobacteriaceae motile, form endospores, or need oxygen - Answer - Either
non-motile or motile with peritrichous flagella
- Do NOT form endospores
- Facultative anaerobes
Characteristic physiology of Enterobacteriaceae - Answer Ferment glucose, reduce
nitrate, catalase positive, oxidase negative
What is Enterbacteriaceae main metabolic activity for differentiating bacteria? - Answer
Ferment or do not ferment lactose
MacConkey Agar (MAC) (2) functions? - Answer - Isolates (selects) and differentiates
Enterobacteriaceae based on their ability to ferment lactose
- Bile salts and crystal violet inhibit the growth of gram-positive organisms
What differentiates the organisms on MacConkey Agar (MAC)? - Answer Lactose and
peptone and neutral red (pH indicator) differentiate the organisms
MacConkey Agar (MAC) (2) outcome? - Answer 1. If an organism ferments lactose,
colony pH will drop below 6.8, and turn pink/red
2. If the organism does NOT ferment lactose, it will use the peptone and remain
colorless/white
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