ACS BIOCHEMISTRY EXAM
QUESTIONS AND ANSWERS
Above the Tm Cholesterol: - Answer-decreases fluidity by limiting the free rotation of bulky
Fatty acid side chains with its bulky structure, effectively decreasing entropy.
Below the Tm Cholesterol: - Answer-increases the fluidity by preventing the F...
The concentration of G and C changes with each genus, - Answer✔✔-the Tm can aid in
identifying unknown bacterial species
DNA replication - Answer✔✔-occurs a lot in the skin, GI and marrow but not much in other
places
non coding - Answer✔✔-introns (98.5%)
coding - Answer✔✔-exons (1.5%)
Topoisomerase - Answer✔✔-unwinds the chromatin structure (tertiary)
Helicase - Answer✔✔-unwinds the helix (secondary)
RNA primase - Answer✔✔-synthesizes RNA primers necessary to the chemistry of replication
single stranded binding proteins (ssbp) - Answer✔✔-stabilize the single strand by blocking
hydrogen bonding. small cationic proteins that stabilize the negatively charged phosphate back
bone
stop DNA from rewinding
DNA replicase - Answer✔✔-enzyme that produces DNA chains
Ligase - Answer✔✔-links okazaki fragments
proof reading enzymes - Answer✔✔-...
repair enzyme - Answer✔✔-not limited to replication, always repairing
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