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MB (ASCP) Exam Questions and Answers |100% Pas

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MB (ASCP) Exam Questions and Answers |100% Pass Predominant form of DNA - Answer-Right-handed B DNA and histone interaction is due to what type of bond? - Answer-Ionic What stains to the minor groove of DNA duplex? - Answer-SYBR Green What is the most abundant type of RNA? - Answer-Ribosomal R...

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  • October 15, 2024
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EMILLECT 2024/2025 ACADEMIC YEAR ©2024 EMILLECT. ALL RIGHTS RESERVED. FIRST PUBLISH OCTOBER 2024.




MB (ASCP) Exam Questions and
Answers |100% Pass

Predominant form of DNA - Answer✔✔-Right-handed B


DNA and histone interaction is due to what type of bond? - Answer✔✔-Ionic


What stains to the minor groove of DNA duplex? - Answer✔✔-SYBR Green


What is the most abundant type of RNA? - Answer✔✔-Ribosomal RNA (rRNA)


What does RNA act as in a retrovirus? - Answer✔✔-Genome and mRNA


What increases half-life of mRNA? - Answer✔✔-5' Methyl Cap


What kind of DNA is most likely to be transcriptionally active? - Answer✔✔-Euchromatin


What is the most common type of eukaryotic DNA? - Answer✔✔-Satellite DNA


What specimen has the most DNA according to molecular weight? - Answer✔✔-White Blood

Cells


What amino acids compose histones? - Answer✔✔-Lysine and Arginine


Amino acid that all proteins start with: - Answer✔✔-Methionine


What protects the DNA strand during replication? - Answer✔✔-Single-stranded binding protein



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Leading Strand - Answer✔✔-3' to 5' template


Lagging Strand - Answer✔✔-5' to 3' template


What DNA strand is being copied in transcription? - Answer✔✔-Antisense Strand


First step after newly formed mRNA is made? - Answer✔✔-Addition of poly(A) tail


Every time the chromosome is copied it would become shorter if not for what enzyme? -

Answer✔✔-Telomerase


What polymerase has repair activity? - Answer✔✔-Beta/Epsilon


What polymerase acts on leading strand? - Answer✔✔-Delta


What polymerase acts on lagging strand? - Answer✔✔-Alpha


Which polymerase is used for mitochondrial replication and repair? - Answer✔✔-Gamma


What polymerase binds the 5' end to 3' end of dsDNA? - Answer✔✔-Topoisomerase


Which RNA polymerase is mainly for transcription? - Answer✔✔-RNA Pol II


Which polymerase is involved with mRNA synthesis? - Answer✔✔-RNA Pol II


What inactivates RNAses? - Answer✔✔-Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to

6 hours at 400C


What type of restriction enzyme recognizes palindromic sites? - Answer✔✔-Restriction Enzyme

Type II


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What type of restriction enzyme lacks methylation activity? - Answer✔✔-Restriction Enzyme

Type II


What is necessary to make cDNA? - Answer✔✔-Reverse transcriptase


Number of SNPs in a human genome? - Answer✔✔-11 billion (10^7)


How many genes (base pairs) are in a human haploid genome? - Answer✔✔-3 billion base pairs


The polymerization of acrylamide requires: - Answer✔✔-Catalyst (APS or TEMED) or Light

activation


How does agarose gel pore size relate to concentration? - Answer✔✔-Inversely proportional


What is denatured during a southern blot? - Answer✔✔-Hydrogen bonds (inter-strand bonds)


Melting temperature formula - Answer✔✔-Tm = (4C x GC Pairs) + (2C x AT pairs)


Which is likely to interfere with fluorescent probe? - Answer✔✔-5' G


Proper GC content of primer - Answer✔✔-35-65%


Inhibitors of PCR - Answer✔✔-Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol

Blue


Order to add to a PCR reaction: - Answer✔✔-Master mix > patient sample > positive control >

negative control


Pre Area - Answer✔✔-Positive Pressure



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Post Area - Answer✔✔-Negative Pressure


Which PCR requires two steps for specificity? - Answer✔✔-Nested PCR


Which microorganism is detected by branched DNA amplification? - Answer✔✔-DNA or RNA


Which technique uses RNA, antibodies, and DNA? - Answer✔✔-Hybrid Capture Assay


Use of bisulfite treatment of DNA to determine its pattern of methylation - Answer✔✔-Bisulfite

DNA


What tests are isothermal? - Answer✔✔-NASBA, TMA, & SDA


Which leukemia is associated with FLT3 gene? - Answer✔✔-AML


T-cell gene rearrangement is seen in what malignancy? - Answer✔✔-Sezary Syndrome


Which translocation causes Acute Premyelocytic Leukemia? - Answer✔✔-t 15;17


Ewing Sarcoma - Answer✔✔-t 11;22


Synovial Sarcoma - Answer✔✔-t X;18


Follicular Lymphoma - Answer✔✔-t 14;18


What genes would be screened in a colorectal panel? - Answer✔✔-MSH2 & MLH1


What gene mutation causes a high risk of breast or ovarian cancer? - Answer✔✔-BRCA1 and

BRCA2


What does MSI test for? - Answer✔✔-Mutations in mismatch repair genes (MMR)

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