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BIO lab exam 2 TAMU Questions And Answers 100% Solved | Already Passed
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BIO lab exam 2 TAMU Questions And Answers 100% Solved | Already Passed
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BIO lab exam 2 TAMU Questions And Answers100% Solved | Already Passed
How did multiplex PCR help in our analysis versus just using a single pair of primers? - ✔✔Using a
single pair would not distinguish with a high degree of certainty.
Why are DNA ladders important? - ✔✔determine the sizes of bands in other DNA samples
Homozygote gene - ✔✔one band at a particular loci
Heterozygote gene - ✔✔two bands at a particular loci
The purpose of using SYBR green in the gels? - ✔✔Binds to DNA and fluoresces when exposed to
the right wavelength
Purpose of using the buffer in the electrophoresis chamber? - ✔✔It controls the net charge of
molecules by maintaining the PH neutral
central dogma of gene expression - ✔✔Genes specify the sequence of mRNA which specify
the sequence of amino acids making up all proteins
Transcription - ✔✔DNA is copied into mRNA
, Translation - ✔✔directs the synthesis of proteins
Primary enzyme used in gene expression & regulation lab? - ✔✔B-galactosidase is an enzyme in E.
coli that is encoded by the LacZ gene when E. coli needs to break down lactose
Operator - ✔✔negative regulatory site that the repressor can bind to
CAP site - ✔✔Positive regulation-site for catabolite activator protein (CAP) can bind to
Lac operon genes - ✔✔lacZ - encodes β-galactosidase enzyme, which splits lactose
into monosaccharides
lacY - encodes lactose permease protein, which is a transmembrane "pump" that allows the cell
to import lactose
lacA - encodes transacetylase enzyme
Expression of the LacZ gene causes the production of what enzyme? - ✔✔B-gal
How can ONPG be used to measure optimal growth conditions for the expression of LacZ? -
✔✔Presence of yellow color would mean that B-gal was produced, thus LacZ would be expressed.
LacZ expression is high in what culture? - ✔✔LB+lactose culture
How to calculate activity - ✔✔Absorbance/time*1000
What sugars are the products of the hydrolysis of lactose? - ✔✔Galactose and glucose
When is the repressor bound or unbound to the operon? - ✔✔The repressor is bound to the operator
when lactose is absent.
When lactose is present the repressor is prevented from binding.
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