MCB 2610 Lab Practical 2
simple stain - correct answer ✔single base dye
heat fix, let cool, CV
direct simple stain - correct answer ✔colors the bacteria
negative simple stain - correct answer ✔colors the background leaves
bacteria unstained
gram stain is a differential or simple stain - correct answer ✔differential
gram negative bacteria
color
morphology - correct answer ✔stained pink
thin peptidoglycan layer with outer membrane
gram pos bacteria are more susceptible to certain antimicrobial agents
(penicillin, B-lactam antibiotics) by inhibiting the formation of peptidoglycan.
TRUE OR FALSE - correct answer ✔true
gram positive bacteria
color
morphology - correct answer ✔stains purple
thick peptidoglycan layer
, heat used during staining does cause sporulation. TRUE OR FALSE - correct
answer ✔false, nutrient deprivation causes sporulation
spore stain
used in response for - correct answer ✔certain harsh environmental
conditions
nutrient deprivation
bacteria in lab we spore stained - correct answer ✔C. glutamicum, B. subtilis
list the growth curve stages - correct answer ✔lag exponential stationary
death
growth curve: lag
occurs when - correct answer ✔occurs when first transferred to growth
media and adjusting to new conditions
increases until ready for division
growth curve: exponential
occurs when - correct answer ✔period of growth where cells divide and grow
simple stain - correct answer ✔single base dye
heat fix, let cool, CV
direct simple stain - correct answer ✔colors the bacteria
negative simple stain - correct answer ✔colors the background leaves
bacteria unstained
gram stain is a differential or simple stain - correct answer ✔differential
gram negative bacteria
color
morphology - correct answer ✔stained pink
thin peptidoglycan layer with outer membrane
gram pos bacteria are more susceptible to certain antimicrobial agents
(penicillin, B-lactam antibiotics) by inhibiting the formation of peptidoglycan.
TRUE OR FALSE - correct answer ✔true
gram positive bacteria
color
morphology - correct answer ✔stains purple
thick peptidoglycan layer
, heat used during staining does cause sporulation. TRUE OR FALSE - correct
answer ✔false, nutrient deprivation causes sporulation
spore stain
used in response for - correct answer ✔certain harsh environmental
conditions
nutrient deprivation
bacteria in lab we spore stained - correct answer ✔C. glutamicum, B. subtilis
list the growth curve stages - correct answer ✔lag exponential stationary
death
growth curve: lag
occurs when - correct answer ✔occurs when first transferred to growth
media and adjusting to new conditions
increases until ready for division
growth curve: exponential
occurs when - correct answer ✔period of growth where cells divide and grow
