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MCB 2610 Lab Practical Questions with complete solution 2024/2025

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MCB 2610 Lab Practical Questions with complete solution 2024/2025 MCB 2610 Lab Practical What is the process of contamination control? - correct answer Aseptic technique What are pipettes - correct answer calibrated glass tubes used to measure and dispense small volumes of liquids P...

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  • October 17, 2024
  • 18
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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MCB 2610 Lab Practical
What is the process of contamination control? - correct answer ✔Aseptic
technique


What are pipettes - correct answer ✔calibrated glass tubes used to measure
and dispense small volumes of liquids


Petri dishes - correct answer ✔small plastic bins


Swabs. cuvettes, pasteur pipettes - correct answer ✔sharps container


What color is the bigger vs. smaller micropipettors pipettes? - correct answer
✔Green - bigger
Blue - smaller


What is the purpose of isolating bacteria? - correct answer ✔To establish a
PURE CULTURE, to be able to study to study the physiology and morphology
of a particular bacterial type


What is the quickest and most economical method of bacterial isolation? -
correct answer ✔Streak plate method


What must you use to view items under 100x? - correct answer ✔immersion
oil


What does parfocal mean? - correct answer ✔It means that if you focus the
specimen in one objective, it'll be in approximate focus in all objectives

,Parts of the microscope labeled - correct answer ✔Carrying the microscope:
Back handle and bottom of the scope


Hottest part of the flame in a bunsen burner - correct answer ✔Inner cone


What 4 things must always be done before leaving the lab? - correct answer
✔Dispose of all cultures and plates in the appropriate containers
Clean your bench with Wescodyne
Take off and put away your lab coat
Wash your hands for at least 20 seconds


What is a simple stain? - correct answer ✔Staining procedures that only use
one stain


What is a direct stain? - correct answer ✔A simple stain that colors the
bacteria


What is a negative stain? - correct answer ✔Simple stain that stains the
background but leaves the bacteria unstained


gram stain - correct answer ✔very important differential stain - classifies
bacteria based on their cell envelope structure


What is the purpose of heat fixing? - correct answer ✔1. Denaturing bacterial
enzymes to prevent autolysis
2. Enhances the adherence of bacterial cells to microscope slide

, In Gram stains, why can't Gram negative bacteria retain the crystal violet? -
correct answer ✔Because Gram negative bacteria have an outer membrane
and a thinner layer of peptidoglycan, which are easily depolarized and can
only retain the counterstain (Safranin-pink)


What are the 3 most common stain? - correct answer ✔Gram, capsule, and
spore stain


What are stains made of? - correct answer ✔Usually salts, a few acids or
bases, charged ions.


The ion that is colored contains a chromophore group


What is it called if the chromophore is a positive ion (like methylene blue)? -
correct answer ✔Basic stain


What is it called if the chromophore is a negative ion? - correct answer
✔Acidic stain


What causes endospore formation? - correct answer ✔It's a response to
certain environmental conditions such as depletion of nutrients, heating,
drying, and other unfavorable conditions.


Heat used during staining does not cause sporulation


Procedure for gram stain - correct answer ✔Use a wax pencil!


flood violet - 1 min
WASH

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