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MCDB 101B Final (Everything after midterm 2) 2024 Questions With All Correct Solutions!!
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MCDB 101B Final (Everything after midterm 2) 2024 Questions With All Correct Solutions!!
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Name: Score:68 Multiple choice questions
Term 1 of 68
Non-stop decay
Enzyme that cuts DNA at a specific sequence of nucleotides
Repression of mRNAs lacking stop codons due to mutation or mis-splicing
sequences of amino acids that fold into functional units
label probe, incubate with DBP, gel electrophoresis, detection
Definition 2 of 68
Drosha crops out pre-miRNA stem-loop
Transported into the cytoplasm
Dicer reduces to miRNA
RISC picks up duplex miRNA
Remaining strand serves as the guide in miRISC (contains argonaute protein)
miRNA mechanism
5' cap addition
Direct repression
exon shuffling
,Term 3 of 68
SNPs
Silences genes:
paternally imprinted= paternal allele is silenced
maternally imprinted= maternal allele is silenced
single nucleotide polymorphism (change): one nucleotide at a loci may be mutated, 1 per 1
kb, most common cause of variation
restriction fragment length polymorphisms
Differences among people in their DNA sequences at sites recognized by restriction
enzymes
Small RNAs generated by processing long transcripts. The piRNAs recruit Piwi protein
complexes to transposable element sites in genomic DNA and prevent TE mobilization.
Term 4 of 68
Direct repression
- Prevent binding of basal factors (RNA Pol 2) to promoter, usually with help of co-
repressor.
- Histone modifying enzymes that close the chromatin at promoter
- Activates promoters over long distances, Intervening DNA bends so the enhancer and
promoter can interact
- Detected through deletion analysis
- Flexible orientation/position
Technique for footprinting ribosomes on mRNAs. High throughput sequencing of mRNA
fragments protected by ribosomes can provide information about translation regulation
- Guanine nucleotide added by capping enzyme in reverse orientation to the 5' end of
eukaryotic RNA primary transcript
- Methyl transferases then add methyl groups to form the cap
, Term 5 of 68
Activation domain
Approximately 1000 bp at both ends of a single BAC clone is sequenced. Knowing the two
sequence reads are connected on a single BAC insert allows genome assembly despite the
presence of repeated elements.
Intrinsically disordered domain. Can bind to basal factors or co-activators (can open
chromatin of promoter by degrading nucleosomes)
- Identifies transcriptional start site (+1 position), critical position and orientation
- Contains TATA box approx. 30 nt upstream of start site
production of DNA clones from mRNA sequences using reverse transcription
cDNA library will have genes present for a specific organ
Definition 6 of 68
Allows DNA-binding proteins to interact with other proteins through the leucine zipper
Homodimer: same subunits
Heterodimer: nonidentical subunits
Alternative splicing
Positional cloning
Dimerization domain
Exon junction complex
Definition 7 of 68
manipulation of the genome in order to cure a disease
Focuses on altering the genomes of somatic cells in patients
Basal factors
Gene therapy
cDNA cloning
Non-stop decay
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