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Bio 2060 L - Final Exam 2024/2025
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Bio 2060 L - Final Exam 2024/2025
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Bio 2060 L - Final Exam 2024/2025aseptic technique - Precise Answer ✔✔technique used to prevent contamination
selective media - Precise Answer ✔✔type of media that contains ingredients that allow the growth of
one type of organism and inhibit the growth of another
sodium azide - Precise Answer ✔✔active ingredient inhibits cytochrome oxidase
differential media - Precise Answer ✔✔type of media that contain ingredients that show visual
differences b/w types of organism
MacConkey Agar - Precise Answer ✔✔color change in colonies indicate lactose fermenting bacteria
differential stain - Precise Answer ✔✔two dye stain used for identifying groups
Bacteria Morphology - Precise Answer ✔✔observing shape, morphology and relative size
bacteria arrangement - Precise Answer ✔✔cocci, bacillus, spirochete
gram stain - Precise Answer ✔✔used to classify bacteria by cell wall composition
gram positive - Precise Answer ✔✔purple, thick peptidoglycan layer
gram negative - Precise Answer ✔✔pink, thin peptidoglycan layer
spore stain - Precise Answer ✔✔uses stem to force malachite green into the spore
Bacillus subtilis - Precise Answer ✔✔spore forming bacteria
, motility nitrate - Precise Answer ✔✔microbes interact with tetrazolium in media; color change
swarming motility - Precise Answer ✔✔motile bacteria that have ability to swarm
sugar fermentation - Precise Answer ✔✔glucose, lactose, mannitol, sucrose
positive:
1. color change; green to yellow
acid production
2. gas production
indole production - Precise Answer ✔✔breakdown of AA; tryptone to indole
Kovac's reagent - Precise Answer ✔✔reagent fo indole production
positive: red ring formed on top
MR - Precise Answer ✔✔tests for methyl red
VP - Precise Answer ✔✔a-napthol and KOH
SA +DMN, zinc dust - Precise Answer ✔✔reagents for nitrate reduction
NO3 to NO2 - Precise Answer ✔✔NO2 present
SA+DMN
turns red
NO3 - Precise Answer ✔✔NO3 present
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