Process of Gel Electrophoresis Questions and Answers 100% Pass
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Process of Gel Electrophoresis
Institution
Process Of Gel Electrophoresis
Process of Gel Electrophoresis
Questions and Answers 100% Pass
What is the first step in the gel electrophoresis process?
The first step is to prepare the agarose gel by dissolving agarose powder in buffer solution
and heating it until fully melted.
How is the gel cast for electrophoresis?...
Process of Gel Electrophoresis
Questions and Answers 100% Pass
What is the first step in the gel electrophoresis process?
✔✔The first step is to prepare the agarose gel by dissolving agarose powder in buffer solution
and heating it until fully melted.
How is the gel cast for electrophoresis?
✔✔The melted agarose is poured into a gel casting tray with a comb placed in it to create wells,
and then it is allowed to solidify at room temperature.
What is the purpose of the comb used in gel electrophoresis?
✔✔The comb creates wells in the solidified gel where samples can be loaded for separation.
How are samples prepared before loading into the gel?
✔✔Samples are mixed with a loading buffer that contains a dye and a density agent, ensuring
they sink into the wells and can be tracked during electrophoresis.
What happens when an electric current is applied to the gel?
1
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