Polyacrylamide Gel Electrophoresis Review Questions with Complete Solutions Rated A+
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Polyacrylamide Gel Electrophoresis
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Polyacrylamide Gel Electrophoresis
Polyacrylamide Gel Electrophoresis Review Questions with Complete Solutions Rated A+
Polyacrylamide gels are used when? - Answers Resolving proteins or DNA fragments of nearly similar size
Polyacrylamide gels have a higher resolving power than? - Answers Agarose gels
Formation of the polyacrylam...
Polyacrylamide Gel Electrophoresis Review Questions with Complete Solutions Rated A+
Polyacrylamide gels are used when? - Answers Resolving proteins or DNA fragments of nearly similar
size
Polyacrylamide gels have a higher resolving power than? - Answers Agarose gels
Formation of the polyacrylamide matrix is catalyzed by? - Answers The production of free radicals by
ammonium perfulate in the presence of ammonium persulfate (TEMED).
Bis-acrylamide is added to the polyacrylamide solution to allow? - Answers Crosslinking of
polyacrylamide chains.
The crosslinking of chains results in the formation of? - Answers A porous gel through which nucleic
acids or proteins migrate.
What are two commonly used types of polyacrylamide gels? - Answers The native gel and the SDS gel
Native gel - Answers Proteins migrate through the gel in their native conformation.
Why is native gel not used to determine molecular weight? - Answers Because the proteins vary in terms
of size, shape, and charge, the molecular weight cannot be determined from this type of gel as size is
not the only factor affecting migration rate.
SDS stands for - Answers Sodium Dodecyl Sulfate
SDS - Answers An anionic detergent. Common ingredient in shampoo.
In SDS gels, - Answers Proteins are denatured. They are not in their native-folded conformation.
SDS disrupts - Answers the secondary, tertiary, and quaternary structure of proteins, leaving them in
their linear, primary form.
Beta-mercaptoethanol - Answers Added as a reducing agent to break any disulfide bonds contributing to
higher level protein structures.
Protein samples are often treated with a combination of SDS, beta-mercaptoethanol, and heat to
ensure? - Answers That the protein is completely denatured before the sample is loaded onto the gel.
SDS also coats the unfolded proteins in the sample with a - Answers Uniform negative charge
For SDS, can we estimate the molecular weight? - Answers The proteins migrate through the gel based
on their size and thus molecular weight estimates of the protein sample can be made if an appropriate
molecular weight ladder has been run on the gel as well.
What are an exception to the rule for estimating molecular weights on SDS gels? - Answers Glycosylated
proteins are an exception. The sugar-side chains linked to the polypeptide backbone cause the
glycosylated protein to migrate differently than would be expected for the actual molecular weight of
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