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PCOL 440 Exam 2 Questions With Correct Answers
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PCOL 440 Exam 2 Questions With Correct Answers Polyclonal antibodies are generated from a single clone of antibody-producing immune cells. - answerFalse The active agents in snake bite antivenoms are antibodies. - answerTrue Monoclonal antibodies recognize many epitopes of the same antigen. - ...

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  • October 20, 2024
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©SIRJOEL EXAM SOLUTIONS
12/3/2024 11:27AM



PCOL 440 Exam 2 Questions With Correct
Answers


Polyclonal antibodies are generated from a single clone of antibody-producing immune cells. -

answer✔False


The active agents in snake bite antivenoms are antibodies. - answer✔True


Monoclonal antibodies recognize many epitopes of the same antigen. - answer✔False


With respect to hybridoma technology, identify the FALSE statement.




- Allows for detailed studies of an antigen's distribution

- Allows for diagnostic characterization of diseases

- Allows for treatment of human disease

- Allows for the generation of gene therapy vectors


- Allows for high degree purification of substances - answer✔Allows for the generation of gene

therapy vectors

, ©SIRJOEL EXAM SOLUTIONS
12/3/2024 11:27AM


Hybridoma technology was developed for the production of polyclonal antibodies. -

answer✔False


With respect to early pregnancy tests (EPTs), identify the False statement.




- Antibodies are used to detect Human Chorionic Gonadotropin (hCG)

- DNA probes are used to detect Human Chorionic Gonadotropin (hCG)

- The ELISA technique is used to detect Human Chorionic Gonadotropin (hCG)

- Human Chorionic Gonadotropin (hCG) is essential in pregnancy

- Human Chorionic Gonadotropin (hCG) production is an early step in pregnancy -

answer✔DNA probes are used to detect Human Chorionic Gonadotropin (hCG)


Which laboratory technique does not use monoclonal antibodies - answer✔Southern blot


Polyclonal antibodies are mixtures of antibodies that recognize different epitopes of the same

antigen. - answer✔True


NIH has guidelines for scientific rigor and reproducibility that grantees are expected to follow -

answer✔True


Monoclonal antibodies are generated from a single clone of antibody-producing immune cells -

answer✔True

, ©SIRJOEL EXAM SOLUTIONS
12/3/2024 11:27AM


Identify the FALSE statement




- ELISA is an acronym for enzyme linked immunosorbent assay

- ELISAs are used to detect antibody-antigen interactions

- In an ELISA, experimental signal is detected by substrate being catalyzed to colored product by

an enzyme linked to the antibody

- ELISA assays have no clinical utility and are only used in basic research laboratories

- Antigens are any molecule or molecular structure that stimulates the immune response -

answer✔ELISA assays have no clinical utility and are only used in basic research laboratories


Using the allelic frequencies of the different STR loci in the associated table, calculate the

probability of an individual within the population having the following genotype.




Calculations made using 2 significant figures:

D7S820: 11,11

F13A01: 6,7


LPL: 10,11 - answer✔~ 1 in 1,000

, ©SIRJOEL EXAM SOLUTIONS
12/3/2024 11:27AM


Using the allelic frequencies of the different STR loci in the associated table, calculate the

probability of an individual within the population having the following genotype. (2 pts)




D7S820: 7, 10

THO1: 7, 9.3

TPOX: 8, 8


Calculations made using 2 significant figures: - answer✔~1 in 3,000


Antibodies can be used therapeutically to provide passive immunity to




- Alzheimer's Disease

- Snake bites

- Infectious diseases

- Diabetes


- Cancer - answer✔Snake bites


Infectious diseases

The estimated cost of misidentified cell lines in biomedical research is approximately: -

answer✔$1 billion

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