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DNA Purification and Recombinant DNA Technology Exam Questions and Answers Already Passed $7.99   Add to cart

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DNA Purification and Recombinant DNA Technology Exam Questions and Answers Already Passed

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DNA Purification and Recombinant DNA Technology Exam Questions and Answers Already Passed Cell Lysis - Answers Method to break down cell membrane to release intercellular materials like DNA, RNA, and proteins Lysate - Answers Preparation obtained after lysing a cell population High Pressure Hom...

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  • October 21, 2024
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  • DNA Purification and Recombinant DNA Technology
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DNA Purification and Recombinant DNA Technology Exam Questions and Answers Already Passed

Cell Lysis - Answers Method to break down cell membrane to release intercellular materials like DNA,
RNA, and proteins

Lysate - Answers Preparation obtained after lysing a cell population

High Pressure Homogenizer (HPH) - Answers Uses high pressure to force material through a narrow
outlet, applying strong shear forces

Bead Mill - Answers Disrupts cells by agitating tiny beads made of glass, steel, or ceramic at high speeds

Thermolysis - Answers Involves repeated freeze/thaw cycles of cells to disrupt cell membranes

Detergent Lysis - Answers Utilizes surfactants to break hydrophobic and hydrophilic interactions of the
cell membrane

Proteinase K - Answers Degrades everything except DNA/RNA in DNA isolation

Phenol-chloroform extraction - Answers Method using alcohols and chloroform to break down proteins
in lysate

Plasmid DNA - Answers Small, circular, double-stranded DNA separate from chromosomal DNA in
prokaryotes and archaea

Miniprep - Answers Method combining alkaline lysis and column chromatography for plasmid isolation

Agarose Gel Electrophoresis - Answers Method to estimate DNA size, analyze PCR products, and purify
DNA

Ethidium Bromide - Answers Common stain that intercalates DNA, used for visualization

DNA Ladder - Answers Standard markers used to determine DNA fragment sizes on a gel

SDS-PAGE - Answers Polyacrylamide Gel Electrophoresis used for protein analysis and small DNA
fragments

Resolution - Answers Electrophoresis resolution measures how well a system can separate molecules of
similar size. Determined by running voltage and agarose concentration.

BACE Q - Answers To achieve the best resolution of PCR products ranging from 200-400bp on an agarose
gel, use a higher percentage of agarose and a higher running voltage.

DNA Quantification - Answers Purity of DNA sample tested with a spectrophotometer. A 260 nm reading
of 0.51 au and a 280 nm reading of 0.65 au. Purity range is 1.8-2.0.

Recombinant DNA Technology - Answers Involves combining DNA sequences not naturally found
together to study genes on a controllable scale and insert DNA into various organisms.

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