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DNA Repair, PCR, and DNA Sequencing Overview Questions and Answers

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DNA Repair, PCR, and DNA Sequencing Overview Questions and Answers Excision Repair - Answers Repair mechanism removing damaged DNA segments. Mismatch Repair - Answers Corrects mismatched bases in newly synthesized DNA. Double-Strand Break - Answers Severe DNA damage requiring specialized repair...

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  • October 21, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • DNA Repair, PCR, and DNA Sequencing
  • DNA Repair, PCR, and DNA Sequencing
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DNA Repair, PCR, and DNA Sequencing Overview Questions and Answers

Excision Repair - Answers Repair mechanism removing damaged DNA segments.

Mismatch Repair - Answers Corrects mismatched bases in newly synthesized DNA.

Double-Strand Break - Answers Severe DNA damage requiring specialized repair mechanisms.

NHEJ - Answers Error-prone repair of double-strand breaks.

HDR - Answers Accurate repair using homologous DNA as template.

Base-Excision Repair - Answers Corrects single damaged bases in DNA.

Nucleotide-Excision Repair - Answers Repairs damage spanning multiple DNA bases.

Restriction Endonucleases - Answers Enzymes cleaving DNA at specific sequences.

Sticky Ends - Answers Overhanging ends of DNA after cleavage.

Blunt Ends - Answers Straight cut ends of DNA after cleavage.

Molecular Cloning - Answers Techniques to create populations of recombinant DNA.

Sanger Sequencing - Answers Method using ddNTPs to determine DNA sequences.

dideoxynucleosides - Answers Chain-terminating nucleotides used in sequencing.

Plasmid Vector - Answers DNA molecule used to clone foreign DNA.

DNA Polymerase - Answers Enzyme synthesizing new DNA strands.

RNA Primer - Answers Short RNA segment initiating DNA synthesis.

dNTPs - Answers Building blocks for DNA synthesis (dATP, dTTP, etc.).

Gel Electrophoresis - Answers Technique for separating DNA fragments by size.

Transgenic Models - Answers Organisms engineered to carry foreign genes.

Gene Therapy - Answers Treatment method delivering genes to correct disorders.

Thymine Dimer - Answers DNA damage caused by UV light.

Cancer - Answers Disease characterized by uncontrolled cell division.

PCR - Answers Technique amplifying specific DNA sequences.

DNA Template - Answers Strand serving as a guide for synthesis.

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