H Biology: Gel Electrophoresis Questions (Quiz SG) Already Graded A
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H Biology: Gel Electrophoresis
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H Biology: Gel Electrophoresis
H Biology: Gel Electrophoresis
Questions (Quiz SG) Already Graded A
What makes DNA think it’s in a race during gel electrophoresis?
The electric current applied to the gel gives DNA a nudge, and since DNA is negatively
charged, it dashes toward the positive side like it’s racing to the fi...
H Biology: Gel Electrophoresis
Questions (Quiz SG) Already Graded A
What makes DNA think it’s in a race during gel electrophoresis?
✔✔The electric current applied to the gel gives DNA a nudge, and since DNA is negatively
charged, it dashes toward the positive side like it’s racing to the finish line!
Why do the smaller DNA fragments win the "gel race"?
✔✔Smaller fragments are like speedy sprinters—they easily slip through the tiny spaces in the
gel, leaving the larger fragments behind.
If the gel were a maze, how would DNA find its way?
✔✔DNA navigates the maze by following the pull of the electric field, with smaller pieces
zooming through the gaps more quickly than larger ones.
Why does DNA glow under UV light, almost like it's "glowing in the dark"?
✔✔The dye (like ethidium bromide) that binds to DNA loves UV light, so when the gel is placed
under UV rays, the DNA lights up like neon signs!
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, What’s the science behind the “comb” in gel electrophoresis? Is it styling the DNA?
✔✔The comb creates little wells where DNA can settle before its journey through the gel, giving
it a neat starting place—no styling necessary!
Why is the buffer solution like a lifeguard for DNA during electrophoresis?
✔✔The buffer keeps everything safe by conducting electricity and maintaining the perfect
environment for DNA to swim through the gel.
Imagine your DNA had too much caffeine. What might happen if you run the gel too fast?
✔✔If you crank up the voltage too high, the DNA might zoom through too quickly, creating
blurry bands that look like your DNA had a caffeine overdose.
How does the gel concentration act like a bouncer at a club for DNA?
✔✔A higher concentration gel is stricter—it makes it harder for larger DNA fragments to
squeeze through, letting only the smaller ones in faster.
What would happen if the DNA "got lost" and never left the well?
✔✔If there’s no current or the loading dye isn’t working, your DNA might stay stuck in the well,
refusing to enter the race altogether!
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