Electrophoresis Steps Latest Update
Graded A+
What is the first step in preparing for gel electrophoresis?
The first step is to prepare the agarose gel by dissolving agarose powder in a buffer solution
and heating it until it melts.
How do you make sure the gel has wells for loading samp...
Gel Electrophoresis Steps Latest Update
Graded A+
What is the first step in preparing for gel electrophoresis?
✔✔The first step is to prepare the agarose gel by dissolving agarose powder in a buffer solution
and heating it until it melts.
How do you make sure the gel has wells for loading samples?
✔✔You insert a comb into the gel mold while the agarose is still liquid; once it cools, the comb
creates wells for the samples.
Why is it important to let the gel solidify before running the experiment?
✔✔The gel needs to solidify to form the matrix through which the DNA will travel; a liquid gel
won’t separate the DNA properly.
What do you add to the DNA samples before loading them into the wells?
✔✔You add loading dye to the DNA samples to make them sink into the wells and track their
progress through the gel.
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, How are the DNA samples loaded into the wells?
✔✔The DNA samples are carefully pipetted into the wells, making sure they don’t spill into the
buffer or adjacent wells.
What’s the next step after loading the DNA samples into the wells?
✔✔Once the samples are loaded, you connect the gel to a power supply, and electricity is
applied to start the separation process.
Why is a buffer added to the gel chamber?
✔✔The buffer surrounds the gel and conducts electricity, allowing the DNA to move through the
gel during electrophoresis.
What happens when the power supply is turned on?
✔✔The electric current causes the negatively charged DNA fragments to move through the gel
toward the positive electrode.
Why do DNA fragments of different sizes separate in the gel?
✔✔Smaller fragments move faster and travel farther through the gel, while larger fragments
move more slowly and stay closer to the wells.
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