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ASCP Clinical Chemistry I Review (youlazy) Exam Questions and Answers 100% Pass
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ASCP Clinical Chemistry I Review (youlazy) Exam Questions and Answers 100% Pass Which of the following lamps provides a continuous spectrum of radiant energy in the visible, near IR, and near UV regions of the spectrum? A. Tungsten-filament B. Hydrogen C. Deuterium D. Mercury vapor - A most...

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  • October 24, 2024
  • 186
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
ASCP Clinical Chemistry I Review

(youlazy) Exam Questions and Answers

100% Pass


Which of the following lamps provides a continuous spectrum of radiant

energy in the visible, near IR, and near UV regions of the spectrum?

A. Tungsten-filament

B. Hydrogen

C. Deuterium

D. Mercury vapor - ✔✔A most common light source for photometry in the

visible region. continuous spectrum (360-800 nm). near IR and UV.



Hydrogen and Deuterium (200-375 nm)

Mercury vapor- not a continuous spectrum, emitting radiation at specific

wavelengths

Which of the following isolates light

within a narrow region of the spectrum?

A. Photomultiplier tube

,©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
B. Monochromator

C. Photovoltaic cell

D. Detector - ✔✔B Use for selecting a narrow band wavelengths from a

continuous spectrum. Three kinds (prisms, filters, diffraction gratings.

Which of the following is not descriptive of a photomultiplier tube?

A. Emits electrons proportionally to

initial light absorbed

B. Must be shielded from stray light

C. Cannot be used with a chopper

D. Amplifies the initial signal received - ✔✔C

PMT- responds to the radiant energy light it absorbs by emitting electrons

in a proportional amount to the initial light absorbed.

Which of the following is false about

a photomultiplier tube?

A. Converts radiant energy (light)

to electrical energy (current)

B. Amplifies the current significantly

C. Has a very rapid response time

D. Is composed of an iron plate and

a layer of selenium - ✔✔D is part of a barrier layer cell

,©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
PMT- 1. light to electricity

2. Amplification

5. Which type of photodetector employs a linear arrangement that allows it

to respond to a specific wavelength resulting in complete UV/visible

spectrum analysis?

A. Photomultiplier tube

B. Phototube

C. Barrier layer cell

D. Photodiode array - ✔✔D

256-2048 arranged in a linear fashion

Respond to a specific wavelength that results in a continuous UV/ visible

spectrum.

When performing spectrophotometer

quality assurance checks, what is the

holmium oxide glass filter used to assess?

A. Linearity

B. Stray light

C. Absorbance accuracy

D. Wavelength accuracy - ✔✔D

In spectrophotometric analysis, what is the purpose of the reagent blank?

, ©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
A. Correct for interfering chromogens

B. Correct for lipemia

C. Correct for protein

D. Correct for color contribution of the reagents - ✔✔D

In regard to bichromatic analysis, which of the following is false?

A. Absorbance is measured at the

spectral absorbance peak for a blank

and the sample using the same

wavelength.

B. Eliminates background interferences

C. Sample concentration determined

from difference in two measured

absorbances

D. Functions as a reference blank for

each sample - ✔✔A

The bandpass of a spectrophotometer is

10 nm. If an instalment is set at 540 nm, the wavelengths that are permitted

to impinge on the sample will be within what

wavelength range?

A. 530-540 nm

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