Genetics 133 Quiz 4 with
100% correct answers 2025
Annealing - answer The pairing of complementary DNA strands to form
double-stranded DNA. This also refers to the formation of DNA-DNA or
RNA-RNA double-stranded helices.
Antiparallel - answer refers to the opposite polarities of the two strands
in a DNA double helix.
Bacteriophage (phage) - answer A virus that infects bacteria
Capsid - answer The outer shell of a virus. Transduction results from DNA
of an infected bacterial host being packaged within the bacteriophage
capsid.
Chromosome - answer A continuous DNA molecule encompassing a linear
array of genes and intergenic sequences. Humans have 23 chromosome
pairs
Cloning (gene) - answer The production of many copies of a gene or
specific DNA sequence.
Cloning Vector - answer The plasmid or phage DNA used to carry and
propagate the cloned DNA.
Conjugation - answer The mating of two bacterial cells during which
chromosomal or plasmid DNA is transferred from the donor to the
recipient cell.
Conjugative plasmid - answer A plasmid that confers the ability to
conjugate (maleness). e.g. F factor
, DNA Ligase - answer The enzyme that joins the ends of two strands of
DNA together to make a continuous DNA strand
DNA polymerase - answer The enzyme that replicates DNA. DNA
polymerase unwinds double-stranded DNA and synthesizes a new
complementary DNA strand using the rules of base pairing
Fertility Factor (F factor) - answer A bacterial plasmid whose presence
condones donor ability (maleness). F factor can exist as a plasmid or
integrated into the host bacteria's chromosome
F+ cell - answer In E. coli, a cell having a free fertility factor; a male cell
that can be a donor in conjugation
F- cell - answer In E. coli, a cell having no fertility factor; a female cell
that can be a recipient in conjugation
Gel Electrophoresis - answer Method for fractioning DNA, RNA, or protein
based on size
Genetically Modified Organisms (GMOs) - answer A popular term for a
transgenic organism, usually referring to an agricultural plant or organism
Hfr (high frequency of recombination cell) - answer In E. coli, a cell
having its F factor integrated into the bacterial chromosome. Hfr cells are
donors (males)
Homologous recombination - answer Genetic exchange between identical
or nearly identical DNA sequences. Homologous recombination involved
the physical breaking and rejoining two double-stranded DNAs.
Horizontal Gene Transfer - answer Gene transfer between bacteria via
natural transformation by naked DNA, transduction by bacteriophages, or
sexual transfer by conjugation
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