Exam (elaborations)
ASCP MB: Extra-Practice() Questions with Answers
- Course
- Institution
ASCP MB: Extra-Practice() Questions with Answers
[Show more]
Seller
Follow
Content preview
ASCP MB: Extra-Practice(2024-2025) Questions withAnswers
Based on the data presented in the table above and with a
prior probability (PP) of 0.5, the probability of paternity
(POP) in this case is:
92.5%
99.5%
90.5%
95.5% - <<,Answers>>>92.5%
This restriction enzyme "digests and removes a methyl group
from Adenine": - <<,Answers>>>Type I
Which method uses 2 pairs of primers to amplify same target
in 2 separate reactions? - <<,Answers>>>nested PCR; highly
specific
useful when specimens are limited in quantity and quality
What method is uses dilutions of known positive standards to
determine relationship between starting target copy/cell
number and cycle number at which fluorescence crosses a
threshold (Ct) - <<,Answers>>>qPCR
,ASCP MB: Extra-Practice(2024-2025) Questions with
Answers
qPCR usually uses _____ fluorescence and can measure
_____. - <<,Answers>>>SYBR Green; copy number of
genes, viral load, tumor load, effects of treatment
What method exhibits fluorescence when the fluorophore is
removed from a quencher when hairpin structure is overcome
by hybdrization of primer sequence? -
<<,Answers>>>scorpion
Branched DNA is a type of _____ amplification. It is used for
quantitative and qualitative detection of _____. -
<<,Answers>>>signal; hepatitis B, C and HIV-1
Denatured target nucleic acid is bound to capture probes fixed
to a plate well. Extender/preamplifier probes then bind to
captured target via complementary binding. Alkaline
phosphatase-labeled nt bind to each extender probe.
Dioxetane added as a substrate for alkaline phosphatase.
Chemiluminescence is measured. This method is _____. -
<<,Answers>>>bDNA
What will increase yield/lower stringency in PCR? -
<<,Answers>>>decrease: annealing temp, extension temp,
GC content
increase: annealing time, extension time, # cycles, MgCl2
,ASCP MB: Extra-Practice(2024-2025) Questions with
Answers
What will decrease nonspecific binding/higher stringency in
PCR? - <<,Answers>>>decrease: annealing time, extension
time, # cycles, MgCl2
increase: annealing temp, extension temp
Formamide _____ Tm by disrupting H-bonds. -
<<,Answers>>>lowers
Denaturing HPLC is used for what analysis and what does it
detect? - <<,Answers>>>heteroduplex analysis; detects
polymorphisms/SNPS
What disease is commonly associated with EBV? -
<<,Answers>>>post-transplant lymphoproliferative disease
(PTLD)
Translocations can be detected by _____. -
<<,Answers>>>karyotype, Southern blot, FISH, PCR, RT-
PCR
Use _____ tubes for blood collection for most nucleic acid
assays involving enzymatic treatment of sample. -
<<,Answers>>>EDTA and/or ACD
, ASCP MB: Extra-Practice(2024-2025) Questions with
Answers
If RNA is your target, you must use special tubes containing
_____ for samples. - <<,Answers>>>RNA stabilizing agents
Viral RNA in whole blood should be stored at ____ (temp). -
<<,Answers>>>Room temp
Human RNA in blood/bone marrow should be processed
within _____ (time frame). - <<,Answers>>>24 hours
Purified isolated DNA stable at RT for _____ in fridge or
_____ in freezer. - <<,Answers>>>several months to a year
at least 10 years
Purified RNA is stable at _____ for up to a year or at _____
for several months. - <<,Answers>>>-80C; -20C
M. tuberculosis is resistant to _____. -
<<,Answers>>>isoniazid, rifampin and streptomycin.
CYP2C9*1 metabolizes warfarin _____. -
<<,Answers>>>normally
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller jackbundi9071. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $10.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
78252 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now