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MB (ASCP) Exam | Latest 2024 With 100% Correct Answers

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MB (ASCP) Exam | Latest 2024 With 100% Correct Answers

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  • October 29, 2024
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MB (ASCP) Exam | Latest 2024 With 100% Correct
Answers

Which RNA polymerase is mainly for transcription? - ✔✔RNA Pol II




Which polymerase is involved with mRNA synthesis? - ✔✔RNA Pol II




What inactivates RNAses? - ✔✔Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to
6 hours at 400C



What type of restriction enzyme recognizes palindromic sites? - ✔✔Restriction Enzyme Type II




What type of restriction enzyme lacks methylation activity? - ✔✔Restriction Enzyme Type II




What is necessary to make cDNA? - ✔✔Reverse transcriptase




Number of SNPs in a human genome? - ✔✔11 billion (10^7)




How many genes (base pairs) are in a human haploid genome? - ✔✔3 billion base pairs




The polymerization of acrylamide requires: - ✔✔Catalyst (APS or TEMED) or Light activation

,How does agarose gel pore size relate to concentration? - ✔✔Inversely proportional




What is denatured during a southern blot? - ✔✔Hydrogen bonds (inter-strand bonds)




Melting temperature formula - ✔✔Tm = (4C x GC Pairs) + (2C x AT pairs)




Which is likely to interfere with fluorescent probe? - ✔✔5' G




Proper GC content of primer - ✔✔35-65%




Inhibitors of PCR - ✔✔Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol Blue




Order to add to a PCR reaction: - ✔✔Master mix > patient sample > positive control >
negative control



Pre Area - ✔✔Positive Pressure




Post Area - ✔✔Negative Pressure




Which PCR requires two steps for specificity? - ✔✔Nested PCR

, Which microorganism is detected by branched DNA amplification? - ✔✔DNA or RNA




Which technique uses RNA, antibodies, and DNA? - ✔✔Hybrid Capture Assay




Use of bisulfite treatment of DNA to determine its pattern of methylation - ✔✔Bisulfite DNA




What tests are isothermal? - ✔✔NASBA, TMA, & SDA




Which leukemia is associated with FLT3 gene? - ✔✔AML




T-cell gene rearrangement is seen in what malignancy? - ✔✔Sezary Syndrome




Which translocation causes Acute Premyelocytic Leukemia? - ✔✔t 15;17




Ewing Sarcoma - ✔✔t 11;22




Synovial Sarcoma - ✔✔t X;18




Follicular Lymphoma - ✔✔t 14;18




What genes would be screened in a colorectal panel? - ✔✔MSH2 & MLH1

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