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Bio 219 Final Exam: Questions & Answers (Guaranteed Pass!)
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Bio 219 Final Exam: Questions & Answers (Guaranteed Pass!)
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Bio 219 Final Exam: Questions & Answers (GuaranteedPass!)
Two Part Purpose of class Right Ans - -create a complete Aliivibrio fischeri
genomic library
-create a restriction map of lux containing plasmid
Aliivibrio fischeri Right Ans - -gram negative
-rod shaped
-chromosomal DNA contains lux operon
Two aspects of gram-negative bacteria Right Ans - -external LPS layer
(lipopolysaccharide)
-single layer of peptidoglycan based cell wall
Operon Right Ans - -genetic regulatory system in which genes coding for
functionally related proteins are clustered along the DNA
Beneficial aspects of bioluminescence Right Ans - -communication
-attracting prey
-hiding from predators
-attracting a mate
-reliable food source for bacteria (glucose + amino acids)
Two types of organisms that use bioluminescence Right Ans - -pinecone
fish
-hawaiian bobtail squid
Quorum Sensing Right Ans - -regulation of gene expression in response to
changes in cell-population density
Auto-induction Right Ans - -bacteria release a small metabolic product, N-
Acyl homoserine lactone, which diffuses across membrane into extracellular
environment
What two things need to be oxidized for fluorescence to occur? Right Ans -
-aldehyde
-flavin mononucleotide
, Luciferase Right Ans - -enzyme that catalyzes the light emitting reaction
-has alpha and beta subunits
Lux I Right Ans - -encodes for auto-inducer
Lux R Right Ans - -encodes for transcriptional activator that binds the auto
inducer
-transcribed oppositely all other subunits
Lux C Right Ans - -encodes for the acyl-reductase enzyme
Lux D Right Ans - -encodes for the acyl-transferase enzyme
Lux A Right Ans - -encodes for the alpha subunit of luciferase - has the
active site for enzyme
Lux B Right Ans - -encodes for the beta subunit of luciferase - absolutely
necessary for the activity of enzyme
Lux E Right Ans - -encodes for acyl-protein synthetase enzyme
Acyl-transferase (lux D) Right Ans - -removes fatty acids from their normal
biosynthetic pathway for use in this reaction
Acyl-Protein synthetase (luxE) Right Ans - -"activates" the fatty acid to
form R-CO-AMP
-ATP dependent
Acyl-reductase (lux C) Right Ans - -reduces the activated fatty acid to form
the necessary aldehyde
-NADPH dependent
Resuspension buffer (Buffer ATL) Right Ans - -contains sodium dodecyl
sulphate (SDS)
-detergent disrupts lipid bilayer of gram negative cells and brings proteins
into solution
-SDS helps release the DNA binding proteins by denaturing them and binding
both membrane and non-membrane proteins as monomers
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