Exam (elaborations)
Microbiology LAB Exam -1 Questions and Answers 2024
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Microbiology LAB Exam -1
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Microbiology LAB Exam #1Microscope- The Condenser - answer focuses the light ging into the objective lens
Microscope- The Iris Diaphragm - answer adjusts the amount of light going into the
objective lens
Microscope- Nosepiece - answer holds the objective lenses and rotates to change from
one objective to another
Microscope- Coarse Objective Knob - answer moves the stage larger distances to help
get the object in focus
Microscope- Fine Adjustment Knob - answer moves the stage smaler increments to
help get the object in better focus
Microscope- Objective Lens - answer magnifies the object. It is located near the object.
Microscope- Occular Lens - answer magnifies the image of the object from the objective
lens. It is located near the eye.
Steps in using the microscope - answer1) scanner power in place
2) place slide of specimen stage in place with stage clips
3) Using the course adjustment knob, raise the stage all the way up and then down
slowly until you can see image of specimen
4) Readjust with fine adjustment knob
Describe the purpose of the oil immersion lens - answerUsed when oil is added to the
slide. Oil must touch the object and objective lens to that the light does not refract futher
Parfocal - answerParfocal microscopes are able to move from one objective to the next
and still be in focus due to the length of the objective lenses
Resolution - answerThe ability to distinguish between two objects that are very close
together.
What is resolving power? - answerThe measurement of resolution
What are two ways to increase resolving power? - answer1. By decreasing the
wavelength of light (using a blue filter) 2. Increasing the numerical aperature
What units are used to express resolving power? - answernm or nanometers
, What is better, smaller or larger resolving power? - answerSmaller is better
Light microscopes have a 200nm resolving power
Electron microscopes have a .2nm resolving power
What is chromatic aberration? - answerThe effect when many colors are seen toward
the edge of the microscope field of vision (like a prisim)
How are microscopes corrected for chromatic aberration? - answerWith a special
achromatic lens (corrected for red and blue light) or by using a blue filter. The lab has
blue filters in their microscopes which also increase resolution.
Describe the process of making a wet-mount slide - answer1) Add the specimen to the
slide
2) Touch the edge of the cover slip with one edge of drop of specimen.
3) Let the cover slip drop gradually to the slide, covering the drop of specimen.
Describe the purpose of making a wet-mount slide - answerTo be able to see living
organisms under the microscope in their natural state.
What is the advantage of using a wet-mount slide? - answerCan see movement and
determine if microbes posses their own method of motility. Can also see the actual
shape and arrangement of microbes.
What is the dis-advantage of using a wet-mount slide? - answerThe movement is very
fast, microbes are transparent. Must use a "dark-field stop".
What is a producer? - answerAre autotroph organisms that make their own food
(through photosynthesis).
What structure is an indicator of a producer? - answerChloroplasts is an indicator that
an organism is a producer.
An example of a producer is what? - answerAlgae are producers that are found in a
pond.
What is a consumer? - answerThey are heterotrophic organisms that get their food from
another source (nonphotosynthetic)
Examples of a consumer are what? - answerProtozoans such as paramecium and
multicellular animals like rotifers or daphnia.
What is osmosis? - answerThe diffusion of water
What organelle of protozoans responds to osmosis? - answerThe cell membrane is
where diffusion occurs.
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