Exam (elaborations)
BCH5413 Exam 1 Questions With Complete Solutions
- Course
- Institution
BCH5413 Exam 1 Questions With Complete Solutions
[Show more]
Content preview
BCH5413 Exam 1 Questions With Complete Solutions1. Choosing an E. coli protein expression system has a lot of
advantages such as yield, cost and ease.
Discuss (not just list) two reasons why a researcher might not
choose this type of system.
Be specific, what might their goal be and why wouldn't this
system work for them? Correct Answer 1. no post-translational
modification - E. coli system is not sophisticated enough for
downstream experimental goals
2. limited cDNA size / codon usage differences - limit the vector
choice for preferred protein
--- "experimental difficulty" = protein expression may not
always be the same between E. coli + mammals
1. In a PCR reaction, why do you need:
a. Both forward + reverse primer
,b. Template
c. dNTP
d. Magnesium
e. DNA polymerase Correct Answer a. provide a "free" 3'-OH
group to which the DNA
polymerase can add dNTPs
b. to make copies of
c. building block nucleotides
d. co-factor = increase DNA polymerase in replicating the
template DNA
e. add nucleotides to new strand
1. In cDNA library construction:
a. What is cDNA?
b. why would an investigator want to create a cDNA library?
,c. what is the advantage of using beads containing oligo T
nucleotides for isolating the RNA? Correct Answer a.
complementary DNA - made via reverse transcriptase
b. to obtain a set of clones representing all mRNAs expressed in
a given cell type
c. oligo T nucleotides = primer to bind to the 3' end poly(A) tail
of most eukaryotic mRNAs
1. In gel electrophoresis:
a. why does DNA move toward the positive pole?
b. why does DNA move according to size? Correct Answer a.
DNA = negative charge move towards positive charge
b. smaller fragments move faster through matrix towards
positive charge
1. In Maxam & Gilbert Sequencing: (chemical modification +
cleavage)
, a. Why do you need to radioactively label the fragment that is
being sequenced?
b. Why do you need to chemically modify the bases?
c. Why do you need an polyacrylamide gel?
d. Why is this method considered direct as compared to other
methods? Correct Answer a. To read the sequence on the gel
b. So that the DNA can be cleaved at SPECIFIC bases
c. Allows DNA to be separated by size by a SINGLE nucleotide
d. the DNA to be sequenced is broken up into readable
fragments
1. In the experiment described that supports DNA as the genetic
material:
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Classroom. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $17.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
79373 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now