100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
BIOC*2580 Midterm Exam Study Guide Solutions $12.49   Add to cart

Exam (elaborations)

BIOC*2580 Midterm Exam Study Guide Solutions

 0 view  0 purchase
  • Course
  • Prep Tests
  • Institution
  • Prep Tests

BIOC*2580 Midterm Exam Study Guide Solutions Partition Chromatography - ANSWER-Separation Stationary phase: solid (silica gel) can H-bond polar AA Mobile phase: solvent flows past particles Polar move slower, non-polar move faster Polarity Thin Layer Chromatography - ANSWER-Silica gel in thi...

[Show more]

Preview 2 out of 8  pages

  • November 3, 2024
  • 8
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • Prep Tests
  • Prep Tests
avatar-seller
KaylinHoffman
Copyright © KAYLIN 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH NOVEMBER, 2024




BIOC*2580 Midterm Exam Study Guide

Solutions


Partition Chromatography - ANSWER✔✔-Separation


Stationary phase: solid (silica gel) can H-bond polar AA


Mobile phase: solvent flows past particles


Polar move slower, non-polar move faster


Polarity


Thin Layer Chromatography - ANSWER✔✔-Silica gel in thin layer


Lower edge has samples, placed in solvent, travels up


Very polar = low Rf, non-polar = high Rf


Polarity


Column Chromatography - ANSWER✔✔-Sample mix added to column


Buffer added, elution volume characteristic of compound


Ion Exchange Chromatography - ANSWER✔✔-Charged stationary phase


Cation exchanger = bind cations


Anion exchanger = bind anions


Elution by competition or pH change, each AA has unique elution volume

Copyright ©Stuvia International BV 2010-2024 Page 1/8

, Copyright © KAYLIN 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH NOVEMBER, 2024


Greater charge = more tightly bonded


Polarity


Metal Affinity Chromatography - ANSWER✔✔-His-tag added to protein gene


His binds tightly to Ni2+, resin has Ni2+


Elution by flooding with imidazole


Gel Filtration or Molecular Exclusion - ANSWER✔✔-Beads with different sized pores


Proteins can enter pores if they fit


Large proteins excluded, travel faster than smaller that can enter


Size


Ultracentrifugation - ANSWER✔✔-Forces cause molecules to sediment at rate dependent on size and

shape


Can determine apparent molecular mass


Size and Shape


Electrophoresis - ANSWER✔✔-Separation based on movement of charged molecules in electric field


Done in gel (polyacrylamide gel)


Size, shape, charge


SDS-PAGE - ANSWER✔✔-SDS-polyacrylamide gel electrophoresis


Protein treated with SDS unfolding it and giving it uniform negative charge


Small proteins fit in pores and move faster than large that take more time to move through gel

Copyright ©Stuvia International BV 2010-2024 Page 2/8

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller KaylinHoffman. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $12.49. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

75057 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$12.49
  • (0)
  Add to cart