Polymerase Chain Reaction Lab Review 2024/2025 with 100% correct answers
2 views 0 purchase
Course
Polymerase Chain Reaction Lab R
Institution
Polymerase Chain Reaction Lab R
We are going to use the blood sample you collected at the crime scene. What needs to happen before the blood sample can be used for PCR? a) DNA has to be isolated from the cells b) No action is needed c) You should shake the tube d) The blood has to be frozen. correct answersa) DNA has to be isolat...
We are going to use the blood sample you collected at the crime scene. What needs to happen before
the blood sample can be used for PCR? a) DNA has to be isolated from the cells b) No action is needed c)
You should shake the tube d) The blood has to be frozen. correct answersa) DNA has to be isolated from
the cells
What do you need to do each time before using pipette to collect liquid? a) Put on a used pipette tip b)
Polish the pipette c) Shake the pipette d) Put on a new, sterile pipette tip correct answersd) Put on a new,
sterile pipette tip
What is the function of primers in a PCR reaction? a) Denature DNA b) Bind specific sites on the DNA c)
Bind random sites on the DNA d) Copy DNA correct answersb) Bind specific sites on the DNA
Why is it important to change the pipette tip? a) To employ more garbage men b) To avoid cross
contamination c) To keep the lab bench clean d) To keep the lab assistant happy correct answersb) To
avoid cross contamination
For which enzyme are nucleotides the substrate? a) DNA polymerase b) Ligase c) Protease d) Ribosome
correct answersa) DNA polymerase
What is the template of the PCR reaction? a) DNA b) RNA c) Nucleotides d) Proteins correct answersa)
DNA
What does a DNA polymerase do? a) Degrades proteins b) Unfolds DNA c) Synthesizes DNA d) Cleaves
DNA correct answersc) Synthesizes DNA
At this step in the PCR process, (95°C) what happens to the DNA? a) It is kept intact b) It will be
separated into two strands c) It will be twisted into double helix d) It will be broken into many pięces
correct answersb) It will be separated into two strands
, How is the DNA separated into single strands? a) The DNA polymerase separating the two DNA strands
b) The high temperature (95 °C) c) The primers separating the two DNA strands d) The low temperature
(54 °C) correct answersb) The high temperature (95 °C)
The area where the primers bind marks which part of the PCR product? a) End b) Beginning C) Left side
d) Right side correct answersb) Beginning
The PCR products get a certain length due to whích fact? a) The DNA breaking off b) The DNA
polymerase falling off C) The heat in the PCR machine d) The placement of the primers correct answersd)
The placement of the primers
How does the DNA polymerase extend the primers into a new DNA strand? a) Adding nucleotides to the
3' and 5' ends of the primers b) Adding nucleotides to the 3' end of the primers c) Adding more primers
to the strand d) Adding nucleotides to the 5' end of the primers correct answersb) Adding nucleotides to
the 3' end of the primers
Primers are always designed to be complementary to the template DNA strand. Which of these
sequences is the complementary sequence to the template sequence 5'-GTGGTCTGATCAACGGTAA-3'? a)
3'-GTGGTCTGATCAACGGTAA-5' b) 3'-CACCAGACTAGTTGCCATT-5' c) 5'-CACCAGACTAGTTGCCATT-3' d) 5'-
GTGGTCTGATCAACGGTAA-3' correct answersb) 3'-CACCAGACTAGTTGCCATT-5'
STR analysis correct answersDifferent length may found in each individual. Since individuals might have
different numbers of repeats creating their own specific DNA profiles.
What happens to the probability of a 100% match between two different individuals when using 13 sets
of primers for the DNA profile instead of one? a) It decreases b) It results in a match c) It increases d) It is
not affected correct answersa) It decreases
How many copies of DNA are required to see bands on the electrophoresis gel? a) 1000 copies b) 10
copies c) Millions of copies d) None correct answersc) Millions of copies
Which word describes the charge of the DNA? a) Positively charged b) Not charged c) Highly charged d)
Negatively charged correct answersd) Negatively charged
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller QUILLSKY. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $13.49. You're not tied to anything after your purchase.
