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BIO 340 FINAL EXAMBANK QUESTIONS ACCURATELY SOLVED FREQUENTLY TESTED.
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BIO 340 FINAL EXAMBANK QUESTIONS ACCURATELY SOLVED FREQUENTLY TESTED.
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BIO 340 FINAL EXAMBANK QUESTIONSACCURATELY SOLVED FREQUENTLY TESTED.
why is endosymbiosis limited to eukaryotes
Correct Answer -eukaryotes are capable of phagocytosis, organelles are not
longer endosymbionts. A BIG distinguishing feature of organelles is
purposeful protein targeting
what process requires endosymbiotic gene transfer and protein targeting?
Correct Answer -genetic integration
restoration enzymes
Correct Answer -restrict the growth of bacteriophages
cut DNA at sequence-specific sites
produce double strand breaks
over 600 commercially available
what cuts DNA at sequence-specific sites
Correct Answer -restoration enzymes
what can cut sites be classified as leaving
Correct Answer --blunt ends or sticky ends
-5' overhang or 3' overhang
what are cut sites generally?
Correct Answer -PALIDROMES
,-dna palindroms have the same sequence on opposite strands
the names if enzymes according to their length in a recognition sequence
Correct Answer -4 cutter
6 cutter
5' overhang
Correct Answer -more letters on the 3' line
3' overhang
Correct Answer -more letters on the 5' line
basic steps of closing overview
Correct Answer -1. cut DNA of interest
2. ligate into vector
3. put vector into organism
4. organism copies plasmid
what are commonly used as vectors during cloning?
Correct Answer -plasmids
vector
Correct Answer -any carrier molecule for the DNA insert of interest
,plasmid
Correct Answer -small, circular, independently replicating, DNA element
found in certain bacteria, organelles, and eukaryotes
why would we want to clone a gene in the first place?
Correct Answer -sequencing and expression
what used two different restriction enzymes
Correct Answer -directional cloning
DNA of interest can only be inserted in..
Correct Answer -one orientation
four major features of plasmid cloning vectors
Correct Answer -1. origin of replication
2. B-lacamase gene
3. multiple cloning sites (MCS)
4.B-galactosidase gene (lacZ)
what color is colonies with NONRECOMBINANT vectors?
Correct Answer -blue
what color is colonies with RECOMBINANT plasmid?
Correct Answer -white
, what can GMOs include?
Correct Answer -hybrids, gammradiated mutants, cloned animals, or any
organisms subjected to selective breeding
what do we really mean by GMO is "genetically engineered organism"?
Correct Answer -an organism to which humans have added a gene or genes
to confer a desired trait (phenotypes)
what is the #1 GM crop worldwide?
Correct Answer -soy
-all of it is engineered for herbicide tolerance, most commonly to glyphosate
what is a pesticide
Correct Answer -any pest killing compound, including herbicide,
insecticides, fungicide
SDS
Correct Answer -soybean sudden death syndrome
what causes SDS
Correct Answer -Fusarium fungus
what does golden rice express?
Correct Answer -beta-carotene synthesis genes in the endosperm
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