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BIO 340 Module 5 Questions And Verified Answers A+ Graded $13.99   Add to cart

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BIO 340 Module 5 Questions And Verified Answers A+ Graded

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BIO 340 Module 5 Questions And Verified Answers A+ Graded...

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  • November 6, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • bio 340 module 5
  • BIO 340 Module 5
  • BIO 340 Module 5
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BIO 340 Module 5 Questions And Verified
Answers A+ Graded


What is the correct order of the enzyme action in DNA replication? Label steps 1 to 7. -
ANSWER 1. Bind to origin of replication (AT rich sequence)

2. Helicase/topoisomerase action

3. Single-stranded binding proteins bind and

block reannealing

4. Priming, or synthesis of RNA primers

5. DNA polymerization/proofreading

6. 5'-3' exonuclease activity/DNA

polymerization

7. Ligation



Transcribe and Translate the following DNA strand

DNA 3' T A C A T A A C C A T T 5' - ANSWER 5' AUGUAUUGGUAA



The haploid human genome is 3 Gbp long (3x10^9 bp). How long would the genomic
DNA in a single diploid cell in your body be if it were stretched out end to end? -
ANSWER -2m



The haploid human genome is 3 Gbp in length (3x109 bp). There are ~5x1013 cells in the
human body. How long would all of the human genomic DNA in your body be if it were
stretch out end to end? - ANSWER 100 billion Km



Over what temperature range does a DNA allow the binding of primers? - ANSWER
45-68

, What are the three major parts of a DNA nucleotide? - ANSWER A phosphate group, a
nitrogenous base, a deoxyribose



Hydrogen bonds are weaker than covalent bonds. Hydrogen bonds are broken in the
process of PCR during the denaturation step. - ANSWER true



T or F about DNA Adenine pairs with Uracil. - ANSWER False



T or F Two strands of DNA Double helix are held together by a weak bond. - ANSWER
True



You are running a PCR and want to estimate about how much DNA you could have after
30 cycles on the thermocycler. - ANSWER 2.14 x 109



What are all the necessary reagents required for the PCR - ANSWER 1. Double stranded
DNA complex with target sequence

2. Deoxyribose nucleoside triphosphate

3. Heat stable DNA polymerase

4. two nonidentical single strand DNA priming sequences (forward and reverse)

5. Buffer



Denaturation - ANSWER heat the mixture to 95° C this will separate the double stranded
DNA into two single strands



Primer Annealing (PCR) - ANSWER the reaction temperature is lowered to 48-68° C. This
allows primers to create bonds with their complementary sequences of the target DNA



Extension/ Elongation - ANSWER the temperature is raised to 72° C. This allows
polymerase to synthesize DNA accurately and rapidly

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