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KSU - BIOCHEM 521, Final Exam Questions With Complete Solutions
- Course
- BIOCHEM 521
- Institution
- Kansas State University
KSU - BIOCHEM 521, Final Exam Questions With Complete Solutions
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KSU - BIOCHEM 521, Final Exam Questions WithComplete Solutions
After the assembly of the snRNPs, the 2 ends of an intron are
brought together. A specific adenine nucleotide in the intron
attacks the 5' splice site and does what? Correct Answer cuts
the sugar-phosphate backbone of the RNA
After transfer of amino groups to a-ketoglutarate forms
glutamate, where is the glutamate transported? Correct Answer
from the cytosol to the mitochondrial matrix
Alanine Correct Answer
alanine + a-ketoglutarate produces: Correct Answer pyruvate +
glutamate
All organisms have 2 tRNAs for methionine, one is used
exclusively when AUG is the what? Correct Answer initiation
codon for protein synthesis
All organisms have 2 tRNAs for methionine, the second one is
used exclusively for what? Correct Answer to code for
methionine in an internal position in a polypeptide
Although methionine has only one codon (AUG), all organisms
have ___ tRNAs for methionine Correct Answer two
Amino acids are transferred through the blood to the liver for
breakdown into what? Correct Answer metabolites that can
enter the citric acid cycle
,Amino groups from all of the amino acids are collected by
transfer to a-ketoglutarate to produce what? Correct Answer
glutamate
Aminoacylation of tRNA is catalyzed by aminoacyl-tRNA
synthase in 2 steps: Correct Answer 1. formation of aminoacyl
adenylate, which remains bound to the active site
2. the aminoacyl group pis transferred to the 3' hydroxyl of the
terminal adenylate in tRNA
An anticodon with I can recognize what? Correct Answer more
than one synonymous codon
Any a-keto acid can be converted to an a-amino acid by what
reaction? Correct Answer transaminase reaction
Aquatic animals ______ NH4+. Correct Answer excrete
Aspartate Correct Answer
aspartate + a-ketoglutarate produces: Correct Answer
oxaloacetate + glutamate
ATP is used in cells as a donor of free energy, and is the what
used by cells? Correct Answer energy currency
Because of the release of a large amount of free energy during
the hydrolysis of phosphoanhydride bonds of ATP, this reaction
is known as what? Correct Answer thermodynamically
favorable, and is often linked to other reactions that have a
,positive delta G, driving such reactions rhrough inout of free
energy
Birds and reptiles convert ammonia to ____ ____. Correct
Answer uric acid
Can acetyl-CoA be converted to pyruvate in animals? Correct
Answer NO; therefore, it is not a precursor for gluconeogenesis
Can amino acids be both glucogenic and ketogenic? Correct
Answer YES
- ex: phenylalanine
Can plants recycle their NH4+? Correct Answer yes, they do
not need to excrete it
Can the carbon skeletons of the essential amino acids be
synthesized by all mammals? Correct Answer no, some
mammals cannot synthesize them
cap binding protein Correct Answer recognizes 7-
methylguanosine cap at the 5' end of the mRNA
Carbs can be linked to asparagine residues in proteins via what
type of linkage? Correct Answer N-linkage
Carbs can be linked to serine and threonine residues by what
type of linkage? Correct Answer O-linkage
Cells require input of what for anabolic pathways and for
motion? Correct Answer free energy
, Coupling of transcription can happen in prokaryotes (bacteria),
but not in eukaryotes, why? Correct Answer because
eukaryotic DNA is found in the nucleus and this is where
transcription occurs; whereas, prokaryotic ribosomes are in the
cytosol and this is where translation occurs
- mRNA stays in the nucleus until mature
Define DNA synthesis: Correct Answer the process of
synthesizing a double stranded DNA through semi-conservative
replication by using enzymes
Define RNA synthesis: Correct Answer the process of
synthesizing an RNA through the process of transcription using
an enzyme-mediated method
Describe the 3' end on a tRNA: Correct Answer contains a
CCA sequence and is the site where the amino acid becomes
covalently attached to tRNA
Disulfide bonds are very common in proteins that are to be..
Correct Answer exported by the cell
DNA polymerase copies an entire molecule, but RNA
polymerase.. Correct Answer only copies the information
contained in one gene
DNA polymerase has proofreading activity, does RNA
polymerase too? Correct Answer NO, so it has a higher error
rate than DNA polymerase
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