Genetic Technology Unit 7 Exam
Questions with Latest Update
Define Human Genome - Answer-The scientific description of the complete set of DNA
in the human organism, including gene locations
Human Genome Project - Answer-an international study of the entire human genetic
material
Goals of Human Genome project - Answer-to map all of the human genes
to construct a detailed physical map of the entire human genome
to determine the nucleotide sequence of all 24 human chromosomes
Where do restriction enzymes come from? - Answer-bacteria
How different restriction enzymes cut DNA at different recognition sites - Answer-they
are coded for a specific sequence in nitrogen bases
Recognition sites - Answer-the specific sites on DNA that restriction enzymes cut
Sticky ends - Answer-overhanging complementary ends that result from a cut by a
restriction enzyme
Blunt cuts - Answer-Enzymes cut strait at the same place on both strands.
Why are restriction enzymes used in genetic technology labs? - Answer-increase the
frequency of desired traits in the population
restriction enzymes - Answer-enzyme that cuts DNA at a specific sequence of
nucleotides
gel electrophoresis - Answer-mixture of DNA fragments is placed at one end of a porous
gel, and an electric voltage is applied to the gel (separating DNA)
The process of preparing a sample to run in a gel step 1 - Answer-restriction enzymes
cut DNA into smaller segments of various sizes
how to interpret multiple samples run on a gel? - Answer-If a sample belongs to an
individual then 100% banding would match up, but if it were a child and a parent 50%
would match up
Various uses for DNA finger printing - Answer-crime scene analysis, paternity testing, to
determine relatedness of organisms, identifying human remains
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