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Genetic Engineering and Recombinant DNA Exam Questions with Complete Answers
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Genetic Engineering and Recombinant DNA Exam Questions with Complete Answers
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Genetic Engineering and RecombinantDNA Exam Questions with Complete
Answers
DNA: The Raw Material - Answer-•Heat-denatured DNA •Strands melt apart
•Slowly cool •Strands reform
•Annealing - Answer-•Re-connection of strands
•Dependent upon salt concentration and percent identity
Enzymes: Restriction Endonucleases - Answer-•Enzymes that cut DNA
•Each has a known sequence of 4 to 10 pairs as its target
•Can recognize and clip at palindromes
•Madam, I'm Adam
•GAATTC
•CTTAAG
BamHI - Bacillus amyloliquefaciens - Answer-G|GATCC
CCTAG|G
EcoRI - Escherichia coli - Answer-G|AATTC
CTTAA|G
EcoRV - Escherichia coli - Answer-GAT|ATC
CTA|TAG
Restriction Endonucleases - Answer-•Can be used to cut DNA in to smaller pieces for
further study or to remove and insert sequences
•Can make a blunt cut or a "sticky end"
•The pieces of DNA produced are called restriction fragments
•Differences in the cutting pattern of specific restriction endonucleases give rise to
restriction fragments of differing lengths - restriction fragment length polymorphisms
(RFLPs)
Ligase: - Answer-Enzyme necessary to seal sticky ends together
Reverse transcriptase: - Answer-enzyme that is used when converting RNA into DNA
•Copied DNA is referred to as complementary DNA or cDNA
Analysis of DNA - Answer-•Gel Electrophoresis
•Electric gradient made
•DNA passes through agarose gel
•Small fragments move fastest
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