Exam (elaborations)
Bio 219 Quizzes Questions And Answers Latest Update
- Course
- BIO 219
- Institution
- Purdue University
Bio 219 Quizzes Questions And Answers Latest Update
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Bio 219 Quizzes Questions AndAnswers Latest Update
In the Hawaiian bobtail squid (Euprymna scolopes) what is the benefit to the
squid in hosting the Aliivibrio?
Communication
b.
Attracting a mate
c.
Attracting prey
d.
Hiding from predators Answer: d
Wash Buffer 2 (AW2) contains ________________ to remove the salts added
from the first wash buffer.
a.hydrochloric acid
b.SDS
c. Guanidinium chloride
d. Ethanol
e. sodium benzoate Answer: d
Which gene in the lux operon encodes the auto-inducer, AHL
Lux R
,b.
Lux I
c.
Lux A
d.
Lux D Answer: b
What is the overall purpose of the quarter-long project?
I. To map the lux operon
II. To isolate the lux operon from A. fischeri and transform E. coli with it the
operon to induce bioluminescence
III. To induce the upregulation of the gadA gene in E. coli through quorum sensing
Answer: 2 and 3
Regulation of gene expression in response to changes in cell population density is
known as _______________________. Answer: quorum sensing
If you wanted to get a brighter bioluminescence from your culture of Allivibrio
which of the following "treatments" would increase the amount of blue-green
light emitted?
treatment with CO2
b.
Treatment with chaotropic salts
c.
treatment with O2
, d.
treatment with acid
e.
Treatment with a alkaline buffer Answer: c
What is used to elute the DNA from the silica membrane in the spin column?
a.
Chaotropic salts
b.
SDS (sodium dodecy sulfate)
c.
Water
d.
Ethanol
e.
A low pH buffer. Answer: c
Good minions always wear their [x] and [y] for lab safety! Answer: gloves
goggles
What is the range for the 260:280 ratio that indicates a DNA sample of "good
purity"?
2.2 - 3.5
b.
0.5 - 1.5
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