BIO 340 module 5 Exam Questions And Answers
What is the correct order of enzyme action during DNA replication? Number the steps from 1 to 7. - answers1. Binding to origin of replication (AT rich sequence)
2. Helicase/topoisomerase activity
3. Single-stranded binding proteins bind and
pre...
What is the correct order of enzyme action during DNA replication? Number the steps from 1
to 7. - answers✔✔1. Binding to origin of replication (AT rich sequence)
2. Helicase/topoisomerase activity
3. Single-stranded binding proteins bind and
prevent reannealing
4. Synthesis of RNA primers (priming)
5. DNA polymerization/proofreading
6. 5'-3' exonuclease activity/DNA polymerization
7. Ligation
Transcribe and Translate the following DNA strand
DNA 3' T A C A T A A C C A T T 5' - answers✔✔5' AUGUAUUGGUAA
The haploid human genome is 3 Gbp long (3x10^9 bp). How long would the genomic DNA
in a single diploid cell in your body be if it were stretched out end to end? - answers✔✔-2m
The haploid human genome is 3 Gbp long (3x10^9 bp). There are approximately 50 trillion
cells in the human body. How long would all of the human genomic DNA in your body be if
it were stretch out end to end? - answers✔✔100 billion Km
At what temperature range does a DNA allow the binding of primers? - answers✔✔45-68
What are the three main components of a DNA nucleotide? - answers✔✔A phosphate group,
a nitrogenous base, a deoxyribose
Hydrogen bonds are weaker than covalent bonds. During PCR, hydrogen bonds are broken
during the denaturation step. - answers✔✔true
T or F regarding DNA Adenine pairs with Uracil. - answers✔✔False
T or F Two strands of DNA Double helix are held together by a weak bond. -
answers✔✔True
You are running a PCR and want to estimate about how much DNA you could have after 30
cycles on the thermocycler. - answers✔✔2.14 x 109
What are all the necessary reagents required for the PCR - answers✔✔1. Double stranded
DNA complex with target sequence
2. Deoxyribose nucleoside triphosphate
3. Heat stable DNA polymerase
4. two nonidentical single strand DNA priming sequences (forward and reverse)
5. Buffer
Denaturation - answers✔✔heat the mixture to 95° C this will separate the double stranded
DNA into two single strands
Primer Annealing (PCR) - answers✔✔the reaction temperature is lowered to 48-68° C. This
allows primers to create bonds with their complementary sequences of the target DNA
Extension/ Elongation - answers✔✔the temperature is raised to 72° C. This allows
polymerase to synthesize DNA accurately and rapidly
the process where the 3' hydroxyl group of the growing chain and the 5' phosphate of a free
nucleoside triphosphate react to form a phosphodiester bond. 5' looses two phosphate to form
this bond. The directionality is important because if these strands are not running antiparallel
the phosphodiester bond will not form. - answers✔✔Elongation
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