WGU D311: Microbiology With Lab:
Section 3 Questions With Answers.
Which factor determines the level of bioburden (microbial level) allowed and, therefore,
the microbial controls required for items used in a general healthcare setting? -
ANSWER- The application or use of the item
Which laboratory biosafety level would be used for managing work with an organism of
moderate risk that is native to the local area? - ANSWER- BSL-2
Which microbial control type can be used to completely eliminate all vegetative bacteria,
endospores, fungi, and viruses from an inanimate item? - ANSWER- Sterilization
Which term describes the area specifically created and maintained for performing
medical procedures that carry risk from contamination? - ANSWER- Sterile field
Which physical method of control works by removing microorganisms from a liquid? -
ANSWER- Filtration
Which physical method of control is used to denature proteins and alter membranes of
microorganisms to prevent spoilage of milk, apple juice, honey, and other ingestible
items to reduce microorganism levels? - ANSWER- Pasteurization
Which technique will ensure that Clostridium botulinum and botulinum toxin cannot
cause foodborne illness? - ANSWER- Boiling foods for at least 10 minutes prior to
eating.
Which method would be used to sterilize an antibiotic solution? - ANSWER- Membrane
filtration
Which chemical treatment would be used before a disinfectant to reach a soiled
surface? - ANSWER- A surfactant
Which class of chemical disinfectant denatures proteins and disrupts membranes? -
ANSWER- Phenolics
Which chemical is commonly used for hand sanitizers and to degerm a site prior to
injections due to its effective destruction of vegetative bacteria by denaturing proteins
and its quick drying properties? - ANSWER- Alcohols
Which chemical is effective at killing bacterial endospores with sufficient time and
concentration? - ANSWER- Peroxygens
Section 3 Questions With Answers.
Which factor determines the level of bioburden (microbial level) allowed and, therefore,
the microbial controls required for items used in a general healthcare setting? -
ANSWER- The application or use of the item
Which laboratory biosafety level would be used for managing work with an organism of
moderate risk that is native to the local area? - ANSWER- BSL-2
Which microbial control type can be used to completely eliminate all vegetative bacteria,
endospores, fungi, and viruses from an inanimate item? - ANSWER- Sterilization
Which term describes the area specifically created and maintained for performing
medical procedures that carry risk from contamination? - ANSWER- Sterile field
Which physical method of control works by removing microorganisms from a liquid? -
ANSWER- Filtration
Which physical method of control is used to denature proteins and alter membranes of
microorganisms to prevent spoilage of milk, apple juice, honey, and other ingestible
items to reduce microorganism levels? - ANSWER- Pasteurization
Which technique will ensure that Clostridium botulinum and botulinum toxin cannot
cause foodborne illness? - ANSWER- Boiling foods for at least 10 minutes prior to
eating.
Which method would be used to sterilize an antibiotic solution? - ANSWER- Membrane
filtration
Which chemical treatment would be used before a disinfectant to reach a soiled
surface? - ANSWER- A surfactant
Which class of chemical disinfectant denatures proteins and disrupts membranes? -
ANSWER- Phenolics
Which chemical is commonly used for hand sanitizers and to degerm a site prior to
injections due to its effective destruction of vegetative bacteria by denaturing proteins
and its quick drying properties? - ANSWER- Alcohols
Which chemical is effective at killing bacterial endospores with sufficient time and
concentration? - ANSWER- Peroxygens
