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CE40246 - Advanced Bioprocess Design - Bioreactor Deisgn for Tissue Engineering Exam
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CE40246 - Advanced Bioprocess Design - Bioreactor Deisgn for Tissue Engineering Exam...
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CE40246 - Advanced Bioprocess Design - Bioreactor Deisgnfor Tissue Engineering Exam
Why is scalable in vitro cell and tissue culture using engineering in vivo-like environment
important?
For the creation of in vitro models for toxicology studies to compare healthy & diseased
tissues. To expand cell populations-e.g., test red blood cells or noval drug. For the
replacement of diseased tissue etc. in the body. The manufacturing of entire organs-e.g.
livers.
Why are bioreactors good?
They provide a controlled environment which allows reproducible data, monitoring due
to biosensors; scalable, cost-efficient & can be automated
At what stage in design should scalability be considered?
the beginning - this allows the same flow patterns on all scales and optimises scale up of
the bioreactors
What needs to be known to design a bioreactor?
in-depth knowledge is required about cells & environment as well as behaviour
what is the best design for a bioreactor
experimental, CFD and mathematical modeling in combination to identofy optimal
operating conditions and scaling
In-vivo
process taking place in a living organism
In-vitro
process taking place in a lab / outside the living organism
How is mass transfer like in culture flasks? - (+ves and -ves)
(+) stiff flat surface
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