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E. coli Transformation Lab Report

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This lab report goes over E. coli transformation

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Saint Leo University
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BIO-330L-CA01









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November 9, 2024
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2024/2025
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E.coli Transformation

Jorge Avila

BIO-330L-CA01

10/3/24

, Abstract:

The objective of the experiment was to apply the pGLO plasmid to transform E.

coli bacteria in order to introduce the gene for ampicillin resistance and Green

Fluorescent Protein (GFP). By introducing foreign DNA into a cell, a process known as

genetic transformation, bacteria can develop new characteristics including antibiotic

resistance and fluorescence. The absorption of the pGLO plasmid by E. coli cells was
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