Which of the four elements in proteins distinguished this class of compounds from
carbohydrates and lipids? - ANSWER Nitrogen
Which of the proteins is water-soluble? - ANSWER Albumin
How do amino acids link to form a chainlike molecule? - ANSWER Peptide bonding
Proteins can be denatured by mechanical agitation, heat, or chemical treatment. What
is meant by denaturation of proteins? - ANSWER Change in tertiary structure
Which aminoacidopathy is NOT due to an enzyme deficiency? - ANSWER Cystinuria
Which of the following aminoacidopathies are due to an enzyme deficiency? - ANSWER
Maple syrup disease, phenylketonuria, and alkaptonuria
How many classes of immunoglobulins are recognized at present? - ANSWER Five
Which of the following reagent is used for serum protein estimation? - ANSWER Biuret
Why should not hemolyzed serum be used in the analysis of total protein? - ANSWER
Hemoglobin is also absorbed at the same wavelength as does protein in the reagent
Which of the following is NOT a characteristic of the biuret reaction? - ANSWER It is
relatively free from interference from lipids and hemoglobin
,Which of the following are true of the biuret reaction? - ANSWER Follows Beer's law for
a reasonable range of protein, dependent upon the presence of at least two peptide
linkages, and insensitive to the low protein levels of urine.
Why does bromcresol purple remain the preferred indicator for albumin dye binding
techniques? - ANSWER There is less interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6? - ANSWER Albumin
The total protein is 7.1 g/dL. The following is the result of serum protein:
Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL
Alpha2 11.0% 0.8 g/dL
Beta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
What is the A/G ratio? - ANSWER 3.8/(.3+.8+1.2+1.1) = 1.1
A serum protein electrophoretic pattern that reveals a beta gamma bridge or broad
gamma is indicative of what disease state? - ANSWER Active cirrhosis
An electrophoretic pattern indicates the following: 30% albumin, 4%-10% alpha-globulin,
4%-10% beta-globulin, and 45% gamma globulin. What condition may exist in the
patient? - ANSWER Monoclonal gammopathy
Glycoproteins and mucoproteins are usually combined with what substance? - ANSWER
Carbohydrates
All of the following are glycoproteins: - ANSWER Ceruloplasmin, folliclestimulating
hormone (FSH), and fibrinogen
, All of the following are glycoproteins EXCEPT: - ANSWER Cryoglobulin
Which one of the following applies to cryoglobulins? - ANSWER They are
temperature-sensitive proteins
Which of the following statements is true of albumin? - ANSWER It is produced in the
liver and is water-soluble
Decreased alpha-fetoprotein values are associated with which condition? - ANSWER
Down syndrome
Which one of the following proteins carries iron? - ANS Transferrin
In acute renal failure, which nonprotein nitrogen increases most rapidly? - ANS Blood
urea nitrogen (BUN)
By the urease method, urea is being enzymatically converted to what end product? -
ANS Ammonia
Why can untreated urine be used for the determination of urea by the diacetyl
monoxomine method? - ANS The method is not measuring ammonia
Which of the following values would most likely accompany an elevated creatinine
value? ANSWER Elevated BUN, 10X creatinine value
What is the classical method for creatinine reaction? ANSWER Jaffe
On what is the measurement of creatinine based, and reagent besides the formation of a
yellow-red color? ANSWER Alkaline picrate
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