Lipid - answer Structural component of cell membrane, Ex: Phospholipid
Covalent bond - answer A chemical bond that involves the sharing of electron pairs
between atoms.
Using too low temp. - answer What is the most likely reason there is an unexpected
band in a PCR reaction?
Molecular weight marker and DNA Ladder - answer What should be included on the gel
to enable you to determine actual sizes of the DNA fragments?
What the order of amino acids on a polypeptide chain is encoded by - answer What is
MRNA?
Function of Quality Assurance (QA) personnel? - answer Who has final say if a
production batch may be released for sale or use?
The 3D structure of DNA is revealed. - answerWhat contributed to biotechnology in the
1950's?
Solid media on which bacteria grow - answerWhat is agar?
R group - answerWhat part of an amino acid differs from one to the other?
Occurs when amino acid sequence is linked by hydrogen bonds. - answerWhat is
secondary protein structure?
Form tetra relevant bonds, highly stable/unstable, able to form short and long chains -
answerWhat are the properties of the carbon atom that make the diversity of carbon
compounds possible?
Unbranched - answerA polypeptide is an ___ chain of amino acids
Hydrophobic - answerAre lipids hydrophilic or hydrophobic?
Carbohydrate - answerStructural component of plant cell walls, Ex: Cellulose
Protein - answerCatalyze biochemical reactions, Ex: Enzyme
Nucleic Acid - answerStore and transfer genetic information in the cell, Ex: RNA
,The nucleotide strands of DNA are oriented in opposite directions in a structure that is
described as - answerWhat is antiparallel DNA?
Restriction enzyme - answerAn enzyme that cuts DNA after recognizing a specific
sequence of DNA.
Phospholipid - answerForm the phospholipid bilayer of the plasma membrane.
Antibody - answerIdentify and neutralize foreign objects such as bacteria and viruses.
Actin/Myosin - answerRules over cell functions which include cell division, morphing of
the shape of the cells, cell mobility and other contractile properties/Helps in muscle
contraction
Enzymes lose function outside of a narrow pH range - answerWhy are enzyme
solutions always prepared using a buffered solvent at a specific pH?
20 - answerHow many amino acids are there?
Leads to increase in high energy collisions. - answerWhy does the rate of a chemical
reaction increase with increasing temperature?
Concentration of reactants, heat, the presence of a catalyst - answerWhat affects rate of
chemical reaction?
Cellular respiration - answerConverts energy into ATP; take place in mitochondria
Aerobic respiration - answerCellular energy with oxygen
Anaerobic respiration - answerCellular energy without oxygen
Process where a single cell divides twice to produce four cells containing half the
original amount of genetic information (sex cells). They go through mitosis. -
answerWhat is meiosis and what happens if it does not happen during sex-cell
development in plants?
What is the purpose of SDS when used in SDS electrophoresis? - answerDenature
proteins and give them negative charge
Haploid: One set of chromosomes; gametes
Diploid: Two sets of chromosomes; somatic cells
Zygote: 2 haploid gametes fuse during fertilization to form a diploid cell (zygote) -
answerWhats difference between diploid/haploid/zygote?
Same as parent; 46 - answerAfter a mitotic division, how many chromosomes do
daughter cells have compared with the parent cell?
,Alleles - answerAlternative forms of a gene that arise by mutation and are found at the
same place on a chromosome.
Diploid - answerAre humans diploid or haploid?
Translation and Transcription - answerGenes encode for proteins. During translation,
MRna encodes a blueprint to make the proteins. In transcription, DNA makes
complimentary RNA.
Semiconservative replication - answerThe replication of DNA, in which each original
strand of DNA serves as a template for a new strand, is called:
A technique used in molecular biology to amplify a single copy or a few copies of a
piece of DNA across several orders of magnitude - answerWhat is polymerase chain
reaction?
Polymerase and Ligase - answerEnzymes involved with in vivo DNA replication, and
which are also used as tools in biotechnology include:
Uracil - answerDNA does not contain base pair
Purines - answerAdenine and guanine
Pyrimidines - answerThymine and cytosine
DNA Polymerase - answer(PCR) requires what enzyme to be thermostable (resistant to
high temperatures) in order to "amplify" a sample of DNA?
Ligase - answerIf you think of recombinant DNA technology as cutting and pasting
pieces of DNA, which molecule represents the glue?
An operon consists of a group of structural genes that code for enzymes involved in a
metabolic pathway. They are under control of a promoter (a short segment of DNA to
which the RNA polymerase binds to initiate transcription) and regulated by an operator.
- answerBacteria cell DNA is divided into operons. Describe an operon using the terms
promoter, operator, and structural gene.
Hydrogen bonding - answerComplimentary base pairing holding two DNA strands
together is due to:
Cell membranes and endoplasmic reticulums - answerWhat are phospholipids found in?
Store the energy we gain from consuming food - answerWhat are polysaccharide useful
for?
, Carbon and hydrogen - answerLipids are composed mostly of what atoms?
Digestion and waste removal - answerWhat is the purpose of lysosomes?
Protista - answerEukaryotic organism that is not an animal, plant or fungus.
4; held together by Peptide bonds - answerHow many polypeptide chains are found in
an antibody, and how are they held together in a protein?
Imitating an infection to produce T cells and antibodies to produce memory t cells so the
body knows how to fight back when actually threatened by this. - answerHow do
vaccines work?
Current good manufacturing practices - answerWhat does cGMP stand for?
All living things are made up of cells, A cell is the smallest unit in a living thing, All cells
come from other cells. - answerWhat is Cell Theory?
Question, hypothesis, experiment, analysis, conclusion - answerList the five steps of
scientific process in order
plasma membranes, phospholipids, one or more chromosones - answerWhat do
eukaryotes and prokaryotes have in common?
nucleus and lysosomes - answerWhat is unique about eukaryotes?
ELISA - answerwhat assay do you run when looking for the presence and concentration
of a specific protein?
SDS Page - answerWhat assay do you run when looking for the identity and purity of a
specific protein?
Time period protein remains active - answerDefine stability.
Spectrophotometer - answerWhat looks for presence of total protein?
Small rings of DNA with only a few genes and floating in the cytoplasm - answerWhat
makes up a prokaryote?
Linear, very long pieces of DNA with many thousands of genes - answerWhat makes up
a eukaryote?
DNA synthesized using an RNA template - answerWhat is CDNA?
TRUE - answerTrue or False: DNA polymerases are the enzymes that create DNA
molecules by assembling nucleotides.
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