Exam (elaborations)
BACE Review (Technical Skills & Applications) Questions and Answers Latest Updated
- Course
- Institution
BACE Review (Technical Skills & Applications)
[Show more]
Content preview
BACE Review (Technical Skills &Applications)
Which of the following should not be used to disinfect an area that is potentially
contaminated with DNA? - answer Alcohol
Why are enzyme solutions always prepared using a buffered solvent at a specific pH? -
answer A buffered solvent is resistant to fluctuations in pH because it is made with a
combination of a weak acid and its conjugate base. Enzymes perform best in solutions
that vary in pH.
Which of the following statements best illustrates the reason for separation of DNA
during gel electrophoresis? - answer DNA fragments are separated in the gel matrix
based on their size.
Which of the following should be included in a gel electrophoresis to determine the size
of the DNA fragment on the gel? - answer Molecular Weight Markers
Gels are used to separate pieces of RNA, DNA, and protein. What type of gel is
typically used for protein electrophoresis? - answer Polyacrylamide
A plasmid has three restriction sites for EcoRI. If the plasmid is treated EcoR1, how
many fragments will be obtained? - answer3
Which of the following can be used as a vehicle for delivering new DNA into a cell? -
answer Plasmid, Lipid and Virus
When developing a new PCR procedure, every variable needs to be tested to determine
the optimum conditions for that specific reaction. Which of the statements best
describes the best experimental design for optimizing the primer-annealing
temperature? - answer Multiple reactions run with Primer, DNA, dNPT's, and a buffer
concentration kept constant, only changing the annealing temperature in a range of 50
to 70 degrees Celsius.
Which of the following enzymes are used to amplify DNA in a Polymerase Chain
Reaction (PCR)? - answer DNA Polymerase
Heat-Stable DNA Polymerase - answer Polymerase Chain Reaction (PCR)
Restriction Enzymes - answer Digesting DNA
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Dreamer252. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $13.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
77858 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now
