SEA-PHAGES Lab Practical UPDATED ACTUAL Questions and CORRECT Answers
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Course
SEA-PHAGES
Institution
SEA-PHAGES
SEA-PHAGES Lab Practical UPDATED
ACTUAL Questions and CORRECT
Answers
During DNA Extraction, how is DNA separated from everything else that may be in the
solution? - CORRECT ANSWER- Nuclease breaks down the bacterial DNA/RNA, but
not the phage's because it is protected by a capsid. Then re...
SEA-PHAGES Lab Practical UPDATED
ACTUAL Questions and CORRECT
Answers
During DNA Extraction, how is DNA separated from everything else that may be in the
solution? - CORRECT ANSWER- ✔✔Nuclease breaks down the bacterial DNA/RNA, but
not the phage's because it is protected by a capsid. Then resin breaks down the capsid.
During DNA Extraction, if the DNA wasn't added at the beginning of the protocol, what
result is very likely? - CORRECT ANSWER- ✔✔The bacterial DNA/RNA would still be
present, so we wouldn't get the pure phage DNA
During streak 3 for purification, if the top agar extremely hot, is it okay to use for plating? -
CORRECT ANSWER- ✔✔No, because if the agar is too hot, it will kill the bacteria.
What is the host used in this lab? - CORRECT ANSWER- ✔✔A strain of bacteria called
Arthrobacter sp. KY 3901
How are the phage samples sterilized in this lab? - CORRECT ANSWER- ✔✔-aseptic
technique to sterilize the area we work in, -filtration to filter the soil sample
-phage buffer to suspend phage particles
What is the difference between sterile and aseptic? - CORRECT ANSWER- ✔✔Sterile is
getting rid of all microorganisms, while aseptic just means "clean technique", or removing
pathogens
Are bacteria Prokaryotes or Eukaryotes? Give 2 characteristics that describe why. -
CORRECT ANSWER- ✔✔Prokaryotes: they reproduce with binary fission, they lack a
nucleus
What is a plaque? - CORRECT ANSWER- ✔✔A plaque is an area on a bacterial lawn that
has been lysed by a phage (clear=lytic phage, cloudy=temperate phage)
, What is the purpose of the plaque streak protocol? - CORRECT ANSWER- ✔✔To isolate a
single type of phage from a plaque
Describe the Lytic life cycle? - CORRECT ANSWER- ✔✔phage attaches itself to cell,
injects DNA to create more phages within the cell, lyses the cell and bursts
Describe the Lysogenic cycle? - CORRECT ANSWER- ✔✔integrates DNA for replication
through bacteria life cycle
If your plates have no plaques, do they need to be recorded in the laboratory notebook? -
CORRECT ANSWER- ✔✔Yes, even negative results need to be recorded
Why do we add the agar to the most dilute area during a streak plate? - CORRECT
ANSWER- ✔✔The most dilute area is the most ideal area to obtain a phage, so we want it to
be pure. If the agar spread from the 1st section to section X, it could be moving phages with
it, making section X not the most dilute
Describe one possible application for a bacteriophage. - CORRECT ANSWER- ✔✔Could be
used in water filtration sites- can lyse bacteria out of water/act as an indicator for
contamination in the water
In Latin, Bacteriophage means - CORRECT ANSWER- ✔✔Eater of bacteria
A bacterium in which a phage has incorporated its DNA into the bacterial genome is called a
... - CORRECT ANSWER- ✔✔Lysogen
If you obtain a plate with 2 different morphologies, is the population cloned? - CORRECT
ANSWER- ✔✔No because it has 2 different morphologies which indicates 2 types of phages
Why would you want to use plants with a web pattern when harvesting your MTL rather than
completely lysed plates? - CORRECT ANSWER- ✔✔In a completely lysed plate, the phage
concentration is too hight. Webbed plates show a more appropriately active phage.
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