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Bio 219- Quizzes Questions With Complete Solutions

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  • BIO 219

Bio 219- Quizzes Questions With Complete Solutions Which gene in the lux operon encodes for the transcriptional activator that binds the auto inducer? a. Lux E b. Lux A c. Lux D d. Lux R e. Lux I D. Lux R The Aliivibrio fischeri strain we are using the term (MJ 1741) comes from the pineap...

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  • November 12, 2024
  • 36
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • BIO 219
  • BIO 219
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Bio 219- Quizzes
Questions With Complete Solutions

Which gene in the lux operon encodes for the transcriptional activator that binds the auto

inducer?

a. Lux E

b. Lux A

c. Lux D

d. Lux R


e. Lux I D. Lux R




The Aliivibrio fischeri strain we are using the term (MJ 1741) comes from the pineapple fish

(Monocentris japonica). Also known as the pineconefish, it is yellow with distinct large scales

outlined in black. The fish is nocturnal and feeds at night. During the day, it shelters in caves and

under ledges. It has light-producing organs filled with the bioluminescent bacteria on each side

of its lower jaw. Though the purpose of these photo-organs is not specifically known, what do

you think may be the benefit to the host organism?

a. Attracting prey

b. Attracting a mate

c.Hiding from predators


d.Communication A. attracting prey

, Bio 219- Quizzes
Questions With Complete Solutions

High level of [x] activates the operon systems of luminous bacteria like A. fischeri in its

environment. The regulatory response to this molecule leads to the induction of expression of the

lux genes which is termed [y]. Therefore, where is the bacteria is most likely to glow? Inside its

host or in the open ocean? [z] x: autoinducer (AHL)


y: quorum sensing

z: inside its host




What is used to elute the DNA from the silica membrane in the spin column?

a. SDS (sodium dodecyl sulfate)

b.Water

c.Ethanol

d.A low pH buffer


e.Chaotropic salts B. water




What is the range for the 260:280 ratio that indicates a DNA sample of "good purity"?

a.0.5 - 1.5

b.2.0 - 2.2

, Bio 219- Quizzes
Questions With Complete Solutions
c.2.2 - 3.5

d.1.8 - 2.0


e.1.5 - 1.8 D. 1.8-2.0




A 260:280 ratio = 1.66 would indicate protein contamination. (True/False) True




If you wanted to get a brighter bioluminescence from your culture of Aliivibrio which of the

following "treatments" would increase the amount of blue-green light emitted?

a.Treatment with acid

b.Treatment with O2

c.Treatment with CO2

d.Treatment with chaotropic salts


e.Treatment with a alkaline buffer B. Treatment with 02




Wash Buffer 1 (AW1) contains low amounts of ________________ that binds to and removes

the proteins and colored contaminants.

a.Ethanol

b.SDS

, Bio 219- Quizzes
Questions With Complete Solutions
c.Guanidinium chloride

d.Sodium benzoate


e.Hydrochloric acid C. Guanidinum Chloride




The Resuspension Buffer contains ____________ that can solubilize the membrane proteins and

disrupt the lipid bilayer of gram-negative cells to help get the proteins into solution. The

phospholipids in the membrane are solubilized by this reagent. It also helps release the DNA by

binding proteins (both membrane and non-membrane proteins) and denaturing them..

A. SBS (sodium benzoate salt)

b.Ethanol

c.Silica

d.SDS (sodium dodecyl sulfate)


e.Chaotropic salts D. SDS (sodium dodecyl sulfate




On the spin column, the formation of an anionic bridge allows DNA to bind to the silica

membrane in the column. (True/False) False




The maximum wavelength of DNA absorbance is 260nm, within the UV range. This is due to the

nitrogenous bases of DNA (adenine, cytosine, guanine, and thymine). At this wavelength, double

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