SEA- PHAGES LAB PRACTICAL WITH
ACTUAL QUESTIONS AND CORRECTLY
WELL DEFINED ANSWERS LATEST 2024 –
2025 ALREADY GRADED A+
Would you classify bacteriophages as living organisms, why or why not? -
ANSWERS-No, they are not living organisms, because they need a host to
reproduce
In the restriction digest procedure, you added the enzymes BamHI to tube 2 and
Clal to tube 3. What might happen if you had added both enzymes to tube 2, and
none to tube 3? - ANSWERS-The fragments will be smaller in tube 2 than in tube 3
Why do we use a 0.22M filter whenever we filter a solution? - ANSWERS-Large
enough to let the liquid filtrate through, but small enough to filter out any solids,
bacteria, or contaminants
What is the host used in this lab? - ANSWERS-A strain of bacteria called
Arthrobacter sp. KY 3901
, How are the phage samples sterilized in this lab? - ANSWERS--aseptic technique
to sterilize the area we work in, -filtration to filter the soil sample
-phage buffer to suspend phage particles
What is the difference between sterile and aseptic? - ANSWERS-Sterile is getting
rid of all microorganisms, while aseptic just means "clean technique", or removing
pathogens
Are bacteria Prokaryotes or Eukaryotes? Give 2 characteristics that describe why.
- ANSWERS-Prokaryotes: they reproduce with binary fission, they lack a nucleus
What is a plaque? - ANSWERS-A plaque is an area on a bacterial lawn that has
been lysed by a phage (clear=lytic phage, cloudy=temperate phage)
What is the purpose of the plaque streak protocol? - ANSWERS-To isolate a single
type of phage from a plaque
Describe the Lytic life cycle? - ANSWERS-phage attaches itself to cell, injects DNA
to create more phages within the cell, lyses the cell and bursts
Describe the Lysogenic cycle? - ANSWERS-integrates DNA for replication through
bacteria life cycle
If your plates have no plaques, do they need to be recorded in the laboratory
notebook? - ANSWERS-Yes, even negative results need to be recorded
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