How does an amplification control help? Correct Answer
Differentiates a true negative from a false negative
Amplification controls have a target that should always
amplify, even if your sample does not amplify
Best molecular assay for Cystic Fibrosis? Correct Answer
CF is caused by a 3b deletion: delF508
RFLP, PCR and Southern Blot are used.
What to use FISH for: Correct Answer PWS/AS can be
detected with FISH
Gaucher - Sequencing or enzyme activity screening
FXS - RFLP, PCR, SSCP
TS - Sequencing or enzyme activity screening
How often should a competency be given/done for
someone in a complex lab? Correct Answer Annually
Important in Final Lab Report in CLIA? Correct Answer
Lab director signature?
Neurotoxin in gel components? Correct Answer Ethidium
Bromide
,What phenomenon is observed in EtBr in gel
electrophoresis? Correct Answer Products slowed
because EtBr alters DNA shape, charge, flexibility
Heteroduplex Correct Answer dsDNA where the
complement strands are not completely complementary
MALDI question something about how it works? Correct
Answer Matrix-assisted laser desorption/ionization, blasts
a surface with sample to produce positive ions that can be
detected.
MALDI-Tof question? Correct Answer Same as MALDI,
but used time of flight (TOF) to differentiate between ions.
Lighter ions travel faster.
Receive a culture flask of fibroblasts, what should you do
next? Correct Answer Options like trypsin and scrape or
pour into tube and centrifuge or just lysis directly in media.
Person guessed.
, Causes of smearing in gel Correct Answer High voltage,
poor sample
Amplification method with polymerase creating a synthetic
dsDNA
Something about getting quality of RNA? answers were all
3 letter acronym with R, like RFN, RIN, something. Correct
Answer RIN is RNA integrity number, compares 28S and
18S RNA molecules. Ratio should be >2
FFPE cervical tissue which molecular method Correct
Answer Line Probe Assay
what type of tube to use for blood sample for molecular
testing in PCR? Correct Answer Options were blood in
EDTA or blood in ACD? Guessing this was a pcr inhibitor
question
EDTA I think is more common.
NGS question about what to do after you've sequenced
Correct Answer Sequence alignment, variant calling,
visualization
A question about what major cause of Prader Willi Correct
Answer Epigenetic, genomic imprinting
IQCP Correct Answer Individualized Quality Control Plan.
Allows labs to develop their own QC for their unique
procedures.
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