A technologist uses the spectrophotometer to quantify the
amount of DNA extracted from a blood specimen diluted
1:30. The absorbance reading at 260 nm was found to be
2.545. The concentration of DNA in
this extract is:
A. 76.35 micrograms/mL
B. 3817.5 micrograms/mL
C. 381.75 micrograms/mL
D. 3054.0 micrograms/mL Correct Answer B (2.545 * 30 *
50 )
50 for DNA
40 for RNA
This enzyme is crucial in the making RNA from DNA:
A. DNA Polymerase
B. RNA Polymerase
C. Helicase
D. Primase Correct Answer B
The nitrogenous base in a nucleotide is expected to be
found on which position of the sugar?
A. C1'
,B. C2'
C. C3'
D. C5' Correct Answer A
Its discovery shed light on why there is simultaneous,
though not continuous, synthesis of DNA on both
leading and lagging strands of DNA:
A. Klenow fragment of DNA polymerase
B. Okazaki fragments
C. Sanger fragments
D. RNA fragments Correct Answer B
The process of making RNA is termed:
A. Replication
B. Translation
C. Transcription
D. Polymerase chain reaction Correct Answer C
The enzyme that primes DNA synthesis is:
A. DNA polymerase
B. Exonuclease
C. DNA ligase
D. DNA primase Correct Answer D
Purines and pyrimidines differ from each other in that:
A. Purines are found RNA; pyrimidines are found in DNA
B. There's no difference between purines and pyrimidines
C. Pyrimidines have two rings; purines have one ring
D. Purines have two rings; pyrimidines have one ring
Correct Answer D
,The phosphate group in a nucleotide is expected to be
found on which position of the sugar?
A. C1'
B. C2'
C. C4'
D. C5' Correct Answer D
All of the following are components of nucleic acids,
EXCEPT:
A. Phosphate group
B. Sugar (ribose or deoxyribose)
C. Nitrogenous base (A, G, C, T)
D. Formamide Correct Answer D
The hydroxyl group in a deoxyribonucleotide is expected
to be found on which position of the sugar?
A. C1'
B. C2'
C. C3'
D. C4' Correct Answer C
In real-time PCR, what is amplified?
A. The target
B. The signal
C. The probe
D. All of the above Correct Answer A
Which of the following is not a PCR step?
A. Denaturation
B. Elongation
C. Annealing
, D. Termination Correct Answer D
In hybrid capture technology, _______
is used to amplify the _______ Correct Answer Alkaline
phosphatase; signal
All of the following methods of amplification are
considered target amplification, except:
A. Quantitative PCR
B. NASBA
C. Strand Displacement Amplification
D. Reverse Transcriptase PCR Correct Answer C
Consider a hypothetical mutation involving gene X. Let's
say you amplify a specific exon, say exon 11, of that gene
then you cut it with restriction enzyme W. In a person
without the mutation, cutting the gene with restriction
enzyme W generates two fragments of sizes, 100 bp and
250 bp. Suppose a C>T mutation in gene X deletes a
restriction site, yielding a fragment of 350 bp. You would
expect a heterozygous person for gene X to
have these fragments on a restriction gel:
A. +/+ = 350 bp; 250 bp; 100 bp;
B. m/+ = Only the 350 bp
C. m/+ = 350 bp; 250 bp; 100 bp
D. m/m = 350 bp; 250 bp Correct Answer C
As a technologist working in a small clinical laboratory, a
nasal swab specimen, suspected to be colonized by a
variety of upper respiratory viruses, came to your
laboratory. You are tasked with determining whether the
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