CITI Introduction to Biosafety Questions
and Answers
This Biosafety Level requires at least two-door separation between the area where
biohazards are used and the personnel not associated with the research, requires
inward inflow into the lab and must have a visual mechanism to verify that air is flowing
in the appropriate direction. - answer BSL3
Ebola virus is an example of a pathogen associated with which Risk Group? - answer
Risk Group 4
The first step in risk assessment for a protocol involving biohazards, the creation of a
formal written risk assessment, should be performed by the: - answer Principal
Investigator
What is the lowest BSL that requires all manipulations of biohazards to take place within
biological safety cabinets or other primary containment enclosures? - answer BSL3
In biosafety, containment is primarily achieved through assessment and establishment
of the following three protective measures: - answer Work Practices, Safety
Equipment, and Facility Design
In Risk Group Comparison Table, Gwladys Caspars' quick guide statement of "don't
touch it refers to biohazards in which Risk Group? - answer Risk Group 2
Which of the following statements is accurate regarding U.S. biohazard regulations? -
answer The OSHA General Duty Clause (Section 5A1) can be applied to biohazards
in the research laboratory.
Which Risk Group is associated with biological materials that pose little or no risk to
healthy adults? - answer Risk Group 1
Which of the following is not a basic component of a medical surveillance program for
workers exposed to biohazards? - answer Confirmation of insurance carrier's
authorization to participate in the project
Which of the following is not an exposure route for a biohazard in the laboratory setting?
- answer Contact of biohazard on hair
What is the first step in the immediate aftermath of an exposure to a biohazard involving
a percutaneous injury (through the skin)? - answer Wash the exposed area
immediately with soap and water for 15 minutes
, It is important to promptly wash the affected area and seek medical assistance following
an exposure to a biohazard. What is time period for administration of HIV post-exposure
prophylaxis? - answer Two hours after the exposure
Which of the following is not a component of the medical surveillance program? -
answer The use of a biological safety cabinet to ensure sterility of research materials
In a study reported in this module on unsafe work practices, researchers were observed
doing this 2.6 times per observation on average while handling human pathogens. -
answer Touching their eyes, nose, or mouth
How many core biosafety work practices (or do's and don'ts) are there, according to the
National Research Council's book entitled, Prudent Practices for the Handling and
Disposal of Infectious Materials? - answer Seven
Which of the following is true about researchers' pets in general? - answer Pets or
animals that are not associated with the research are not permitted in the laboratory
What is the best reason why gloves should be changed periodically while working with
biohazards? - answer Glove integrity will weaken over time with glove use and
stretching
The universal biohazard symbol shall be placed on which of the following items for
labeling to indicate the presence of a potential risk? - answer Laboratory equipment
housing biohazards (such as incubators and freezers)
Which answer is correct regarding sharps containers? - answer Needle boxes or
sharps containers must be located in the immediate vicinity of use and never be allowed
to overfill.
Which statement is a good work practice regarding needles and syringes? - answer
Needles and syringes should never be recapped by hand and must be discarded
directly into a sharps container or needle box located in the immediate work area.
Which of the following would be considered good practice for cleaning up large pieces
of contaminated glass in a laboratory biohazard spill? - answer Wear gloves, a lab
coat, and face protection; use a dust pan and tongs
Which of the following statements is accurate? - answer Reusable sharps must be
placed in a labeled, rigid container containing disinfectant or in a rigid container for
reprocessing to protect handlers until they are decontaminated.
Which dilution of hydrogen peroxide would not be considered a broad spectrum and
sporicidal disinfectant? - answer 0.5 percent (the effective concentration of hydrogen
peroxide is 3.0 to 25.0 percent)
and Answers
This Biosafety Level requires at least two-door separation between the area where
biohazards are used and the personnel not associated with the research, requires
inward inflow into the lab and must have a visual mechanism to verify that air is flowing
in the appropriate direction. - answer BSL3
Ebola virus is an example of a pathogen associated with which Risk Group? - answer
Risk Group 4
The first step in risk assessment for a protocol involving biohazards, the creation of a
formal written risk assessment, should be performed by the: - answer Principal
Investigator
What is the lowest BSL that requires all manipulations of biohazards to take place within
biological safety cabinets or other primary containment enclosures? - answer BSL3
In biosafety, containment is primarily achieved through assessment and establishment
of the following three protective measures: - answer Work Practices, Safety
Equipment, and Facility Design
In Risk Group Comparison Table, Gwladys Caspars' quick guide statement of "don't
touch it refers to biohazards in which Risk Group? - answer Risk Group 2
Which of the following statements is accurate regarding U.S. biohazard regulations? -
answer The OSHA General Duty Clause (Section 5A1) can be applied to biohazards
in the research laboratory.
Which Risk Group is associated with biological materials that pose little or no risk to
healthy adults? - answer Risk Group 1
Which of the following is not a basic component of a medical surveillance program for
workers exposed to biohazards? - answer Confirmation of insurance carrier's
authorization to participate in the project
Which of the following is not an exposure route for a biohazard in the laboratory setting?
- answer Contact of biohazard on hair
What is the first step in the immediate aftermath of an exposure to a biohazard involving
a percutaneous injury (through the skin)? - answer Wash the exposed area
immediately with soap and water for 15 minutes
, It is important to promptly wash the affected area and seek medical assistance following
an exposure to a biohazard. What is time period for administration of HIV post-exposure
prophylaxis? - answer Two hours after the exposure
Which of the following is not a component of the medical surveillance program? -
answer The use of a biological safety cabinet to ensure sterility of research materials
In a study reported in this module on unsafe work practices, researchers were observed
doing this 2.6 times per observation on average while handling human pathogens. -
answer Touching their eyes, nose, or mouth
How many core biosafety work practices (or do's and don'ts) are there, according to the
National Research Council's book entitled, Prudent Practices for the Handling and
Disposal of Infectious Materials? - answer Seven
Which of the following is true about researchers' pets in general? - answer Pets or
animals that are not associated with the research are not permitted in the laboratory
What is the best reason why gloves should be changed periodically while working with
biohazards? - answer Glove integrity will weaken over time with glove use and
stretching
The universal biohazard symbol shall be placed on which of the following items for
labeling to indicate the presence of a potential risk? - answer Laboratory equipment
housing biohazards (such as incubators and freezers)
Which answer is correct regarding sharps containers? - answer Needle boxes or
sharps containers must be located in the immediate vicinity of use and never be allowed
to overfill.
Which statement is a good work practice regarding needles and syringes? - answer
Needles and syringes should never be recapped by hand and must be discarded
directly into a sharps container or needle box located in the immediate work area.
Which of the following would be considered good practice for cleaning up large pieces
of contaminated glass in a laboratory biohazard spill? - answer Wear gloves, a lab
coat, and face protection; use a dust pan and tongs
Which of the following statements is accurate? - answer Reusable sharps must be
placed in a labeled, rigid container containing disinfectant or in a rigid container for
reprocessing to protect handlers until they are decontaminated.
Which dilution of hydrogen peroxide would not be considered a broad spectrum and
sporicidal disinfectant? - answer 0.5 percent (the effective concentration of hydrogen
peroxide is 3.0 to 25.0 percent)
