CBIO 3400 Exam 4 UPDATED ACTUAL Questions and CORRECT Answers
0 view 0 purchase
Course
CBIO 3400
Institution
CBIO 3400
CBIO 3400 Exam 4 UPDATED ACTUAL
Questions and CORRECT Answers
what are the 3 main families of protein filaments - CORRECT ANSWER- actin
microtubules
intermediate filaments
what are the common properties of protein filaments? - CORRECT ANSWER- composed
of polymers
highly regulated by other...
CBIO 3400 Exam 4 UPDATED ACTUAL
Questions and CORRECT Answers
what are the 3 main families of protein filaments - CORRECT ANSWER- ✔✔actin
microtubules
intermediate filaments
what are the common properties of protein filaments? - CORRECT ANSWER- ✔✔composed
of polymers
highly regulated by other factors
can be dynamic or stable
what are the functions of the cytoskeleton? - CORRECT ANSWER- ✔✔provide
shape/structure to the cell
provide connections btwn parts of the cell
what is the main function of the cytoskeleton? - CORRECT ANSWER- ✔✔enable cell
division
how does the cytoskeleton help with cell division? - CORRECT ANSWER- ✔✔cell has
dynamic actin that assemble lamellipodia and filopodia at the leading edge
microtubules assist the polarization of actin
after cell division filaments reorganize
what do neutrophil do? - CORRECT ANSWER- ✔✔in human blood they hunt bacteria
what the 2 microtubule motors? - CORRECT ANSWER- ✔✔kinesin-1 and cytoplasmic
dynein
*they are microtubule motors that transport intracellular cargoes
actin structure - CORRECT ANSWER- ✔✔helical polymer of actin monomers
,can exist in ATP and ADP bound states
polymers are polar
*** plus end = barbed end
minus end = pointed end
actin nucleation - CORRECT ANSWER- ✔✔actin monomers can be assembled into dimers-
trimers-filaments
much more stable as a trimer
nucleation meaning - CORRECT ANSWER- ✔✔creation of first trimer
what are the 2 types of actin monomers - CORRECT ANSWER- ✔✔g-actin
globular
g actin - CORRECT ANSWER- ✔✔globular monomeric form of actin produced in solution
of low ionic concentration
globular
soluble protein
occurs in low ionic concentration
forms the actin filament
f actin - CORRECT ANSWER- ✔✔a fibrous protein polymerized in the form of a double
helix produced in the presence of a metal cation and ATP
filamentous
insoluble
occurs in high ionic concentrations
forms cytoskeleton and contractile apparatus of muscle cells
actin is an... - CORRECT ANSWER- ✔✔ATPase
catalyzes the change from adp to atp
, polymerization - CORRECT ANSWER- ✔✔atp bound subunits are added to polymer and
have high affinity for each other
hydrolysis of ATP not needed
Pi released slowly and causes conformational change that destabilizes the polymer
adp bound actin allows for de-polymerization
the plus and minus end of actin polymerize at different rates - CORRECT ANSWER-
✔✔true
speed of each end of actin - CORRECT ANSWER- ✔✔minus = slow -hydrolysis catches up
plus = fast - hydrolysis lags behind
what can initiate polymerization? - CORRECT ANSWER- ✔✔salt addition
experimental approach to study actin polymerization - CORRECT ANSWER- ✔✔g-actin is
purified to high concentration without polymerization when kept with ATP and low levels of
cations
f/g-actin can be separated with centrifuge
f goes to pellet
ratio determined by western blot
what are 2 other ways to detect f-actin - CORRECT ANSWER- ✔✔nucleotide hydrolysis and
actin polymerization
what is faster dissociation for adp actin or atp actin - CORRECT ANSWER- ✔✔adp
what does nucleotide hydrolysis lead to - CORRECT ANSWER- ✔✔treadmilling - never
stable
what is treadmilling - CORRECT ANSWER- ✔✔when subunits are lost from one end at the
same rate they are being added to the other end
*occurs at actin concentration above Cc for + and below Cc for -
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller MGRADES. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $10.99. You're not tied to anything after your purchase.
