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Week 2 UNE Biochem Exam Questions and Answers
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Week 2 UNE Biochem Exam Questions and Answers
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Week 2 UNE Biochem Exam Questionsand Answers
Michaelis-Menten equation - Answers -vi = (vmax [S])/(Km + [S])
vi - Answers -initial velocity; the initial rate of the reaction at a certain substrate
concentration
Vmax - Answers -the maximal velocity (rate) a reaction can achieve at an infinite
concentration of substrate
Km - Answers -the substrate concentration at which the reaction rate is at half maximum
and is a measure of the substrates affinity for the enzyme
small Km indicates - Answers -high affinity which means that the rate will approach
Vmax at lower concentrations of substrate
[S] - Answers -substrate concentration
deltaG - Answers -presence of an enzyme has no impact on overall energy
rate of reactions can be influenced by - Answers -temperature and hydrogen ion
concentration
oxidation - Answers -loss of electrons
reduction - Answers -gain of electrons
competitive inhibitor - Answers -binds in the active site
for competitive inhibitor, Km - Answers -increases - the substrate concentration has to
be increased to compete with the competitive inhibitor
for competitive inhibitor, Vmax - Answers -remains the same - if the concentration of
substrate is high enough, there is very little chance for the competitive inhibitor to bind
to the active site
when a competetive inhibitor is present... - Answers -enzyme reaction is slower
impact of inhibitor can be overcome by - Answers -an increase in substrate
concentration
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